Developing novel methods to search for substrates of protein kinases such as Rho-kinase

Tomoki Nishioka, Md Hasanuzzaman Shohag, Mutsuki Amano, Kozo Kaibuchi

Research output: Contribution to journalReview articlepeer-review

13 Citations (Scopus)


Protein phosphorylation is a major and essential post-translational modification in eukaryotic cells that plays a critical role in various cellular processes. Recent progresses in mass spectrometry techniques have enabled the effective identification and analysis of protein phosphorylation. Mass spectrometry-based approaches in investigating protein phosphorylation are very powerful and informative and can further improve our understanding of protein phosphorylation as a whole, but they cannot determine the upstream kinases involved. We introduce several studies that attempted to uncover the relationships between various kinases of interest and substrates, including two methods we developed: an in vitro approach termed the kinase-interacting substrate screening (KISS) method and an in vivo approach termed the phosphatase inhibitor and kinase inhibitor substrate screening (PIKISS) method. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases.

Original languageEnglish
Pages (from-to)1663-1666
Number of pages4
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Issue number10
Publication statusPublished - 01-10-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology


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