TY - JOUR
T1 - Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection
AU - Kamachi, Kazunari
AU - Toyoizumi-Ajisaka, Hiromi
AU - Toda, Kohei
AU - Soeung, Sann Chan
AU - Sarath, Svay
AU - Nareth, Ya
AU - Horiuchi, Yoshinobu
AU - Kojima, Kazunobu
AU - Takahashi, Motohide
AU - Arakawa, Yoshichika
PY - 2006/5
Y1 - 2006/5
N2 - We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.
AB - We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.
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U2 - 10.1128/JCM.44.5.1899-1902.2006
DO - 10.1128/JCM.44.5.1899-1902.2006
M3 - Article
C2 - 16672435
AN - SCOPUS:33646540703
SN - 0095-1137
VL - 44
SP - 1899
EP - 1902
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 5
ER -