Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection

Kazunari Kamachi, Hiromi Toyoizumi-Ajisaka, Kohei Toda, Sann Chan Soeung, Svay Sarath, Ya Nareth, Yoshinobu Horiuchi, Kazunobu Kojima, Motohide Takahashi, Yoshichika Arakawa

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.

Original languageEnglish
Pages (from-to)1899-1902
Number of pages4
JournalJournal of clinical microbiology
Volume44
Issue number5
DOIs
Publication statusPublished - 05-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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