Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara; Chihaya Imai; Elaine Coustan-Smith; Jeffrey S. Dome; Massimo Dominici; Elio Vanin; Dario Campana

doi:10.1046/j.1365-2141.2003.04217.x

Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara, Chihaya Imai, Elaine Coustan-Smith, Jeffrey S. Dome, Massimo Dominici, Elio Vanin, Dario Campana

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT⁺ mesenchymal cells continued to grow for > 2 years: parallel TERT^- cultures underwent senescence after 15 weeks. TERT⁺ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34⁺ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT⁺ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

Original language	English
Pages (from-to)	846-849
Number of pages	4
Journal	British Journal of Haematology
Volume	120
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-2141.2003.04217.x
Publication status	Published - 01-03-2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Hematology

Access to Document

10.1046/j.1365-2141.2003.04217.x

Cite this

@article{e4197abddd9640a0b69cafb08c6c9c82,

title = "Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase",

abstract = "To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.",

author = "Keichiro Mihara and Chihaya Imai and Elaine Coustan-Smith and Dome, {Jeffrey S.} and Massimo Dominici and Elio Vanin and Dario Campana",

year = "2003",

month = mar,

day = "1",

doi = "10.1046/j.1365-2141.2003.04217.x",

language = "English",

volume = "120",

pages = "846--849",

journal = "British Journal of Haematology",

issn = "0007-1048",

publisher = "John Wiley and Sons Inc.",

number = "5",

}

TY - JOUR

T1 - Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

AU - Mihara, Keichiro

AU - Imai, Chihaya

AU - Coustan-Smith, Elaine

AU - Dome, Jeffrey S.

AU - Dominici, Massimo

AU - Vanin, Elio

AU - Campana, Dario

PY - 2003/3/1

Y1 - 2003/3/1

N2 - To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

AB - To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

UR - http://www.scopus.com/inward/record.url?scp=0037335705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037335705&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2141.2003.04217.x

DO - 10.1046/j.1365-2141.2003.04217.x

M3 - Article

C2 - 12614220

AN - SCOPUS:0037335705

SN - 0007-1048

VL - 120

SP - 846

EP - 849

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 5

ER -

Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this