Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara, Chihaya Imai, Elaine Coustan-Smith, Jeffrey S. Dome, Massimo Dominici, Elio Vanin, Dario Campana

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

Original languageEnglish
Pages (from-to)846-849
Number of pages4
JournalBritish Journal of Haematology
Volume120
Issue number5
DOIs
Publication statusPublished - 01-03-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology

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