Abstract
To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.
| Original language | English |
|---|---|
| Pages (from-to) | 846-849 |
| Number of pages | 4 |
| Journal | British Journal of Haematology |
| Volume | 120 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-03-2003 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Hematology
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Mihara, K., Imai, C., Coustan-Smith, E., Dome, J. S., Dominici, M., Vanin, E., & Campana, D. (2003). Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase. British Journal of Haematology, 120(5), 846-849. https://doi.org/10.1046/j.1365-2141.2003.04217.x