Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara; Chihaya Imai; Elaine Coustan-Smith; Jeffrey S. Dome; Massimo Dominici; Elio Vanin; Dario Campana

doi:10.1046/j.1365-2141.2003.04217.x

Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Keichiro Mihara
, Chihaya Imai
, Elaine Coustan-Smith
, Jeffrey S. Dome
, Massimo Dominici
, Elio Vanin
, Dario Campana

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

Abstract

To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT⁺ mesenchymal cells continued to grow for > 2 years: parallel TERT^- cultures underwent senescence after 15 weeks. TERT⁺ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34⁺ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT⁺ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

Original language	English
Pages (from-to)	846-849
Number of pages	4
Journal	British Journal of Haematology
Volume	120
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-2141.2003.04217.x
Publication status	Published - 01-03-2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Hematology

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10.1046/j.1365-2141.2003.04217.x

Cite this

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title = "Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase",

abstract = "To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.",

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author = "Keichiro Mihara and Chihaya Imai and Elaine Coustan-Smith and Dome, \{Jeffrey S.\} and Massimo Dominici and Elio Vanin and Dario Campana",

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AU - Mihara, Keichiro

AU - Imai, Chihaya

AU - Coustan-Smith, Elaine

AU - Dome, Jeffrey S.

AU - Dominici, Massimo

AU - Vanin, Elio

AU - Campana, Dario

PY - 2003/3/1

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AB - To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years: parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar. had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.

KW - Acute lymphoblastic leukaemia

KW - Haematopoiesis

KW - Mesenchymal cells

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Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase

Abstract

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