Development of a method to identify persistent and blanchable redness by skin blotting in mice

Ayano Nakai, Takeo Minematsu, Shiori Nitta, Wei Jhen Hsu, Hiromi Tobe, Hiromi Sanada

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Persistent and blanchable redness (PBR) is not currently included in category I pressure injury (PI), which is defined as non-blanchable redness (NBR). However, PBR progresses to PI in a clinical setting. Therefore, it should be clinically managed as category I PI, and a method to distinctly identify PBR is needed. This study aimed to examine whether PI-related biomarkers can distinguish PRB from transient redness (TR) and NBR using skin blotting. TR, PBR, and NBR models were established by the different conditions of dorsal skin compression. Redness observation and skin blotting were performed, and the skin tissue samples were subjected to histological and molecular biological analyses. The vascular endothelial growth factor (Vegf) b, heat shock protein (Hsp) 90aa1, tumour necrosis factor, interleukin (Il) 1b, and Il6 messenger ribonucleic acid levels were significantly different between the three models. The VEGF-A, VEGF-B, IL-1β, and IL-6 protein levels were different between the three models. Although the results of skin blot examinations were inconsistent with those of the expression analysis of tissue, HSP90α and IL-1β are suggested to be potential markers to distinguish PBR from TR and NBR.

Original languageEnglish
Pages (from-to)1168-1182
Number of pages15
JournalInternational Wound Journal
Issue number4
Publication statusPublished - 04-2023

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology


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