Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)–based PCR assay for the detection of Escherichia albertii

Atsushi Hinenoya, Hidetoshi Ichimura, Noritomo Yasuda, Seiya Harada, Kazuhiro Yamada, Masahiro Suzuki, Yoshio Iijima, Akira Nagita, M. John Albert, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Many Escherichia albertii isolates, an emerging pathogen of human and birds, might have been misidentified due to the difficulty of differentiating this bacterium from Escherichia coli and Shigella spp. by routine biochemical tests, resulting in underestimation of E. albertii infections. We have developed a polymerase chain reaction (PCR) assay that targets E. albertii cytolethal distending toxin (Eacdt) genes, which include the genes previously identified as Escherichia coli cdt-II. This assay could generate a single 449-bp PCR product in each of 67 confirmed E. albertii strains but failed to produce PCR product from any of the tested non–E. albertii enteric strains belonging to 37 different species, indicating 100% sensitivity and specificity of the PCR assay. The detection limit was 10 CFU per PCR tube and could detect 105 CFU E. albertii per gram of spiked healthy human stool. The Eacdt gene-based PCR could be useful for simple, rapid, and accurate detection and identification of E. albertii.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume95
Issue number2
DOIs
Publication statusPublished - 10-2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)–based PCR assay for the detection of Escherichia albertii'. Together they form a unique fingerprint.

  • Cite this

    Hinenoya, A., Ichimura, H., Yasuda, N., Harada, S., Yamada, K., Suzuki, M., Iijima, Y., Nagita, A., Albert, M. J., Hatanaka, N., Awasthi, S. P., & Yamasaki, S. (2019). Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)–based PCR assay for the detection of Escherichia albertii. Diagnostic Microbiology and Infectious Disease, 95(2), 119-124. https://doi.org/10.1016/j.diagmicrobio.2019.04.018