TY - JOUR
T1 - Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)–based PCR assay for the detection of Escherichia albertii
AU - Hinenoya, Atsushi
AU - Ichimura, Hidetoshi
AU - Yasuda, Noritomo
AU - Harada, Seiya
AU - Yamada, Kazuhiro
AU - Suzuki, Masahiro
AU - Iijima, Yoshio
AU - Nagita, Akira
AU - Albert, M. John
AU - Hatanaka, Noritoshi
AU - Awasthi, Sharda Prasad
AU - Yamasaki, Shinji
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/10
Y1 - 2019/10
N2 - Many Escherichia albertii isolates, an emerging pathogen of human and birds, might have been misidentified due to the difficulty of differentiating this bacterium from Escherichia coli and Shigella spp. by routine biochemical tests, resulting in underestimation of E. albertii infections. We have developed a polymerase chain reaction (PCR) assay that targets E. albertii cytolethal distending toxin (Eacdt) genes, which include the genes previously identified as Escherichia coli cdt-II. This assay could generate a single 449-bp PCR product in each of 67 confirmed E. albertii strains but failed to produce PCR product from any of the tested non–E. albertii enteric strains belonging to 37 different species, indicating 100% sensitivity and specificity of the PCR assay. The detection limit was 10 CFU per PCR tube and could detect 105 CFU E. albertii per gram of spiked healthy human stool. The Eacdt gene-based PCR could be useful for simple, rapid, and accurate detection and identification of E. albertii.
AB - Many Escherichia albertii isolates, an emerging pathogen of human and birds, might have been misidentified due to the difficulty of differentiating this bacterium from Escherichia coli and Shigella spp. by routine biochemical tests, resulting in underestimation of E. albertii infections. We have developed a polymerase chain reaction (PCR) assay that targets E. albertii cytolethal distending toxin (Eacdt) genes, which include the genes previously identified as Escherichia coli cdt-II. This assay could generate a single 449-bp PCR product in each of 67 confirmed E. albertii strains but failed to produce PCR product from any of the tested non–E. albertii enteric strains belonging to 37 different species, indicating 100% sensitivity and specificity of the PCR assay. The detection limit was 10 CFU per PCR tube and could detect 105 CFU E. albertii per gram of spiked healthy human stool. The Eacdt gene-based PCR could be useful for simple, rapid, and accurate detection and identification of E. albertii.
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U2 - 10.1016/j.diagmicrobio.2019.04.018
DO - 10.1016/j.diagmicrobio.2019.04.018
M3 - Article
C2 - 31272742
AN - SCOPUS:85068226129
SN - 0732-8893
VL - 95
SP - 119
EP - 124
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
ER -
