Development of forensic diagnosis of acute sarin poisoning

Masataka Nagao; Takehiko Takatori; Yoshitaka Maeno; Ichiro Isobe; Hiroyoshi Koyama; Tsukasa Tsuchimochi

doi:10.1016/S1344-6223(02)00086-X

Development of forensic diagnosis of acute sarin poisoning

Masataka Nagao, Takehiko Takatori, Yoshitaka Maeno, Ichiro Isobe, Hiroyoshi Koyama, Tsukasa Tsuchimochi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

On March 20, 1995, the Tokyo subway system was subjected to a horrifying terrorist attack with sarin gas (isopropyl methylphosphonofluoridate) that left 12 persons dead and over 5000 injured. In order to diagnose the definite cause of death of the victims, a new method was developed to detect sarin hydrolysis products in the erythrocytes and formalin-fixed cerebella from four victims of sarin poisoning. Sarin-bound acetylcholinesterase (AChE) was solubilized from the specimens of sarin victims and digested with trypsin. The sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion. The digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Sarin hydrolysis products were detected in all sarin poisoning victims.

Original language	English
Pages (from-to)	S34-S40
Journal	Legal Medicine
Volume	5
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/S1344-6223(02)00086-X
Publication status	Published - 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Issues, ethics and legal aspects

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1344-6223(02)00086-X

Cite this

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title = "Development of forensic diagnosis of acute sarin poisoning",

abstract = "On March 20, 1995, the Tokyo subway system was subjected to a horrifying terrorist attack with sarin gas (isopropyl methylphosphonofluoridate) that left 12 persons dead and over 5000 injured. In order to diagnose the definite cause of death of the victims, a new method was developed to detect sarin hydrolysis products in the erythrocytes and formalin-fixed cerebella from four victims of sarin poisoning. Sarin-bound acetylcholinesterase (AChE) was solubilized from the specimens of sarin victims and digested with trypsin. The sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion. The digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Sarin hydrolysis products were detected in all sarin poisoning victims.",

author = "Masataka Nagao and Takehiko Takatori and Yoshitaka Maeno and Ichiro Isobe and Hiroyoshi Koyama and Tsukasa Tsuchimochi",

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T1 - Development of forensic diagnosis of acute sarin poisoning

AU - Nagao, Masataka

AU - Takatori, Takehiko

AU - Maeno, Yoshitaka

AU - Isobe, Ichiro

AU - Koyama, Hiroyoshi

AU - Tsuchimochi, Tsukasa

PY - 2003

Y1 - 2003

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AB - On March 20, 1995, the Tokyo subway system was subjected to a horrifying terrorist attack with sarin gas (isopropyl methylphosphonofluoridate) that left 12 persons dead and over 5000 injured. In order to diagnose the definite cause of death of the victims, a new method was developed to detect sarin hydrolysis products in the erythrocytes and formalin-fixed cerebella from four victims of sarin poisoning. Sarin-bound acetylcholinesterase (AChE) was solubilized from the specimens of sarin victims and digested with trypsin. The sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion. The digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Sarin hydrolysis products were detected in all sarin poisoning victims.

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Development of forensic diagnosis of acute sarin poisoning

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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