Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita; Tasuku Ueno; Hiroyasu Esumi; Hideyuki Saya; Kazuhiro Kaneko; Katsuya Tsuchihara; Yasuteru Urano

doi:10.1021/acs.bioconjchem.6b00618

Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

Original language	English
Pages (from-to)	302-306
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	28
Issue number	2
DOIs	https://doi.org/10.1021/acs.bioconjchem.6b00618
Publication status	Published - 15-02-2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.bioconjchem.6b00618

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title = "Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells",

abstract = "Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.",

author = "Atsushi Yagishita and Tasuku Ueno and Hiroyasu Esumi and Hideyuki Saya and Kazuhiro Kaneko and Katsuya Tsuchihara and Yasuteru Urano",

note = "Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2017",

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doi = "10.1021/acs.bioconjchem.6b00618",

language = "English",

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journal = "Bioconjugate Chemistry",

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T1 - Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

AU - Yagishita, Atsushi

AU - Ueno, Tasuku

AU - Esumi, Hiroyasu

AU - Saya, Hideyuki

AU - Kaneko, Kazuhiro

AU - Tsuchihara, Katsuya

AU - Urano, Yasuteru

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

AB - Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

UR - https://www.scopus.com/pages/publications/85013087521

UR - https://www.scopus.com/inward/citedby.url?scp=85013087521&partnerID=8YFLogxK

U2 - 10.1021/acs.bioconjchem.6b00618

DO - 10.1021/acs.bioconjchem.6b00618

M3 - Article

C2 - 27976863

AN - SCOPUS:85013087521

SN - 1043-1802

VL - 28

SP - 302

EP - 306

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 2

ER -

Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Abstract

All Science Journal Classification (ASJC) codes

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