TY - JOUR
T1 - Development of mesenchymal stem cells partially originate from the neural crest
AU - Morikawa, Satoru
AU - Mabuchi, Yo
AU - Niibe, Kunimichi
AU - Suzuki, Sadafumi
AU - Nagoshi, Narihito
AU - Sunabori, Takehiko
AU - Shimmura, Shigeto
AU - Nagai, Yasuo
AU - Nakagawa, Taneaki
AU - Okano, Hideyuki
AU - Matsuzaki, Yumi
N1 - Funding Information:
We thank Emi Hiratsu, Miyuki Ogawara, and Takayuki Ohkawa for technical assistance and Lawrence Lein for proofreading this paper. This work was supported by a grant-in-aid from the 21st Century COE program of the Ministry of Education, Science, and Culture of Japan to Keio University.
PY - 2009/2/20
Y1 - 2009/2/20
N2 - Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only been defined in vitro, much of their development in vivo is still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP+ and GFP- fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest.
AB - Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only been defined in vitro, much of their development in vivo is still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP+ and GFP- fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest.
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U2 - 10.1016/j.bbrc.2009.01.031
DO - 10.1016/j.bbrc.2009.01.031
M3 - Article
C2 - 19161980
AN - SCOPUS:58949099180
SN - 0006-291X
VL - 379
SP - 1114
EP - 1119
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -