TY - GEN
T1 - DEVELOPMENT OF MICROCHANNEL IMMUNOASSAY DEVICE APPLYING THE TRANSPARENCY OF CELLULOSE-DERIVED MATERIALS
AU - Shin, Jungchan
AU - Kasama, Toshihiro
AU - Miyake, Ryo
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - In this study, we developed novel microchannel defined by transparent cellophane film (roof), water-resistant paper (bottom), and double-sided tape (sides). The cellophane film enabled us to perform photolithography and microscope observation. By utilizing these features of the cellophane film, we fabricated immunoreaction media called immuno-wall in each microchannel by photolithography. Furthermore, we captured fluorescence images of the immuno-wall through the cellophane film after performing the fluorescence immunoassay. We demonstrated that the sensitivity of immuno-wall devices between cellophane film and water-resistant paper was comparable to that of commercial ELISA kit. Cellophane film is promising material in the next generation microchannel devices.
AB - In this study, we developed novel microchannel defined by transparent cellophane film (roof), water-resistant paper (bottom), and double-sided tape (sides). The cellophane film enabled us to perform photolithography and microscope observation. By utilizing these features of the cellophane film, we fabricated immunoreaction media called immuno-wall in each microchannel by photolithography. Furthermore, we captured fluorescence images of the immuno-wall through the cellophane film after performing the fluorescence immunoassay. We demonstrated that the sensitivity of immuno-wall devices between cellophane film and water-resistant paper was comparable to that of commercial ELISA kit. Cellophane film is promising material in the next generation microchannel devices.
KW - fluorescence sandwich immunoassay
KW - microfluidic channel
KW - paper-based analytical device
UR - https://www.scopus.com/pages/publications/85136999944
UR - https://www.scopus.com/pages/publications/85136999944#tab=citedBy
M3 - Conference contribution
AN - SCOPUS:85136999944
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1413
EP - 1414
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -