Development of Monoclonal Antibodies Reacting against Mycobacterial 65 kDa Heat Shock Protein by Using Recombinant Truncated Products

Isao Hara, Noriyuki Sato, Akihiro Matsuura, Joong‐moon ‐m Cho, Qi Weimin, Toshihiko Torigoe, Thomas M. Shinnigk, Sadao Kamidono, Kokichi Kikuchi

Research output: Contribution to journalArticle

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Abstract

A mycobacterial 65 kDa molecule is a member of the GroEL heat shock protein family. We developed mAbs reacting against recombinant 65 kDa protein by using a gene (pTB12) which encodes this protein. Three mAbs (B20, B97 and B167) reacted selectively with 65 kDa proteins of Mycobacterium tuberculosis, BCG and Mycobacterium leprae, although B20 and B167 may weakly react with a 15 kDa molecule of mammalian cells. One (B108) was obviously cross‐reactive between mycobacterial 65 kDa and the mammalian intracytoplasmic protein. We also developed deletion mutants of pTB12. The localization of these mAb‐defined epitopes was determined by using truncated proteins of the Mycobacterium tuberculosis 65 kDa molecule produced in E. coli. Immunohistochemical analysis showed that B20, B97 and B167 mAbs could detect this antigen in experimental granulomas induced by injection of BCG in the subcutaneous tissue of rats. These mAbs should be useful for analyzing the immunobiologic roles of mycobacterial 65 kDa molecules.

Original languageEnglish
Pages (from-to)995-1007
Number of pages13
JournalMicrobiology and Immunology
Volume35
Issue number11
DOIs
Publication statusPublished - 01-01-1991

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Heat-Shock Proteins
Monoclonal Antibodies
Mycobacterium bovis
Proteins
Mycobacterium tuberculosis
Chaperonin 60
Mycobacterium leprae
Subcutaneous Tissue
Granuloma
Epitopes
Escherichia coli
Antigens
Injections
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Hara, Isao ; Sato, Noriyuki ; Matsuura, Akihiro ; Cho, Joong‐moon ‐m ; Weimin, Qi ; Torigoe, Toshihiko ; Shinnigk, Thomas M. ; Kamidono, Sadao ; Kikuchi, Kokichi. / Development of Monoclonal Antibodies Reacting against Mycobacterial 65 kDa Heat Shock Protein by Using Recombinant Truncated Products. In: Microbiology and Immunology. 1991 ; Vol. 35, No. 11. pp. 995-1007.
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Development of Monoclonal Antibodies Reacting against Mycobacterial 65 kDa Heat Shock Protein by Using Recombinant Truncated Products. / Hara, Isao; Sato, Noriyuki; Matsuura, Akihiro; Cho, Joong‐moon ‐m; Weimin, Qi; Torigoe, Toshihiko; Shinnigk, Thomas M.; Kamidono, Sadao; Kikuchi, Kokichi.

In: Microbiology and Immunology, Vol. 35, No. 11, 01.01.1991, p. 995-1007.

Research output: Contribution to journalArticle

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AU - Kamidono, Sadao

AU - Kikuchi, Kokichi

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