[Development of mRNA quantification after fluorescence activated cell sorting].

Research output: Contribution to journalArticle

Abstract

Recent studies using stem cells or cancer stem cells have revealed the importance of detecting minor populations of cells in blood or tissue and analyzing their biological characteristics. These cells can be separated from other cells by fluorescence-activated cell sorting (FACS) using antibodies that bind to surface antigen; however, the majority of these cells do not have specific surface antigens. Furthermore, the cells must be kept alive throughout the procedure to analyze their biological characteristics. To improve these limitations, we have established a novel laboratory test named mRNA quantification after fluorescence-activated cell sorting(FACS-mQ). Using this method, cells are sorted by a specific gene expression pattern, and the gene expression profile in the sorted cells can be easily analyzed. In this feature, we will introduce the typical applications of FACS-mQ targeting intracellular mRNA and intracellular nuclear antigens. These methods will hopefully contribute to the accumulation of knowledge regarding human stem cells, cancer stem cells, and small populations of cells, the biological characteristics of which are mostly unknown.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalRinsho byori. The Japanese journal of clinical pathology
Volume60
Issue number2
Publication statusPublished - 01-02-2012

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Flow Cytometry
Messenger RNA
Neoplastic Stem Cells
Surface Antigens
Stem Cells
Nuclear Antigens
Transcriptome
Population
Blood Cells
Gene Expression
Antibodies

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "Recent studies using stem cells or cancer stem cells have revealed the importance of detecting minor populations of cells in blood or tissue and analyzing their biological characteristics. These cells can be separated from other cells by fluorescence-activated cell sorting (FACS) using antibodies that bind to surface antigen; however, the majority of these cells do not have specific surface antigens. Furthermore, the cells must be kept alive throughout the procedure to analyze their biological characteristics. To improve these limitations, we have established a novel laboratory test named mRNA quantification after fluorescence-activated cell sorting(FACS-mQ). Using this method, cells are sorted by a specific gene expression pattern, and the gene expression profile in the sorted cells can be easily analyzed. In this feature, we will introduce the typical applications of FACS-mQ targeting intracellular mRNA and intracellular nuclear antigens. These methods will hopefully contribute to the accumulation of knowledge regarding human stem cells, cancer stem cells, and small populations of cells, the biological characteristics of which are mostly unknown.",
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[Development of mRNA quantification after fluorescence activated cell sorting]. / Yamada, Hiroya.

In: Rinsho byori. The Japanese journal of clinical pathology, Vol. 60, No. 2, 01.02.2012, p. 139-144.

Research output: Contribution to journalArticle

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