Deviated Overexpression of TCR-β, TCR-γ, CD4, and CD8 on Thymic Lymphomas Induced by 1-Propyl-1-Nitrosourea

Destruction of the Allelic Exclusion of TCR-β and Expression of Functional TCR-βγ Heterodimer on a Lymphoma, cFTL53

Miyuki Kinebuchi, Akihiro Matsuura, Toshiaki Ogiu, Kokichi Kikuchi

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Abstract

Thymic lymphomas (FTLs) induced by the chemical carcinogen 1-propyl-1-nitrosourea (PNU) in F344 rats showed deviated overexpression of TCR-β, TCR-γ, CD4, and CD8. Even though most FTLs were in the CD4+CD8+ stage, all FTLs expressed TCR-β mRNA with TCR-γ mRNA, but without TCR-α mRNA or TCR-δ mRNA. One of the FTLs, cFTL53, expressed two kinds of TCR-β mRNA and two kinds of TCR-γ mRNA, but did not express any mRNA of TCR-α or TCR-δ. Both alleles of TCR-β loci were rearranged on cFTL53. cDNA cloning and sequencing analysis showed that one TCR-γ mRNA, Vγ4-Jγ1-Cγ1, and both TCR-β mRNA, Vβ2-Dβ2-Jß2.1 and Vβ19-Dβ2-Jβ2.1-Cβ2, on cFTL53 were in the productive form, while the other TCR-γ mRNA, Vγ1-Jγ4-Cγ4L, was not. Both TCR β-chains and a TCR γ-chain were expressed on cFTL53, making a novel set of TCR-βγ heterodimer. Cross-linking of TCR-βγ heterodimer on cFTL53 resulted in a calcium flux, indicating that TCR-βγ works as a signal transduction receptor. Thus, there are four strange phenomena on FTLs; CD4 and CD8 are expressed without TCR-αβ or TCR-γδ, TCR-β mRNA and TCR-γ mRNA were expressed simultaneously without TCR-α and TCR-δ mRNA on FTLs, the allelic exclusion of TCR-β was destroyed in cFTL53, and a novel set of functional TCR-βγ heterodimer was expressed on cFTL53.

Original languageEnglish
Pages (from-to)748-756
Number of pages9
JournalJournal of Immunology
Volume159
Issue number2
Publication statusPublished - 15-07-1997
Externally publishedYes

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propylnitrosourea
Lymphoma
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

@article{6343d262d07f40af9c3a60c1c6ee9e99,
title = "Deviated Overexpression of TCR-β, TCR-γ, CD4, and CD8 on Thymic Lymphomas Induced by 1-Propyl-1-Nitrosourea: Destruction of the Allelic Exclusion of TCR-β and Expression of Functional TCR-βγ Heterodimer on a Lymphoma, cFTL53",
abstract = "Thymic lymphomas (FTLs) induced by the chemical carcinogen 1-propyl-1-nitrosourea (PNU) in F344 rats showed deviated overexpression of TCR-β, TCR-γ, CD4, and CD8. Even though most FTLs were in the CD4+CD8+ stage, all FTLs expressed TCR-β mRNA with TCR-γ mRNA, but without TCR-α mRNA or TCR-δ mRNA. One of the FTLs, cFTL53, expressed two kinds of TCR-β mRNA and two kinds of TCR-γ mRNA, but did not express any mRNA of TCR-α or TCR-δ. Both alleles of TCR-β loci were rearranged on cFTL53. cDNA cloning and sequencing analysis showed that one TCR-γ mRNA, Vγ4-Jγ1-Cγ1, and both TCR-β mRNA, Vβ2-Dβ2-J{\ss}2.1 and Vβ19-Dβ2-Jβ2.1-Cβ2, on cFTL53 were in the productive form, while the other TCR-γ mRNA, Vγ1-Jγ4-Cγ4L, was not. Both TCR β-chains and a TCR γ-chain were expressed on cFTL53, making a novel set of TCR-βγ heterodimer. Cross-linking of TCR-βγ heterodimer on cFTL53 resulted in a calcium flux, indicating that TCR-βγ works as a signal transduction receptor. Thus, there are four strange phenomena on FTLs; CD4 and CD8 are expressed without TCR-αβ or TCR-γδ, TCR-β mRNA and TCR-γ mRNA were expressed simultaneously without TCR-α and TCR-δ mRNA on FTLs, the allelic exclusion of TCR-β was destroyed in cFTL53, and a novel set of functional TCR-βγ heterodimer was expressed on cFTL53.",
author = "Miyuki Kinebuchi and Akihiro Matsuura and Toshiaki Ogiu and Kokichi Kikuchi",
year = "1997",
month = "7",
day = "15",
language = "English",
volume = "159",
pages = "748--756",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
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TY - JOUR

T1 - Deviated Overexpression of TCR-β, TCR-γ, CD4, and CD8 on Thymic Lymphomas Induced by 1-Propyl-1-Nitrosourea

T2 - Destruction of the Allelic Exclusion of TCR-β and Expression of Functional TCR-βγ Heterodimer on a Lymphoma, cFTL53

AU - Kinebuchi, Miyuki

AU - Matsuura, Akihiro

AU - Ogiu, Toshiaki

AU - Kikuchi, Kokichi

PY - 1997/7/15

Y1 - 1997/7/15

N2 - Thymic lymphomas (FTLs) induced by the chemical carcinogen 1-propyl-1-nitrosourea (PNU) in F344 rats showed deviated overexpression of TCR-β, TCR-γ, CD4, and CD8. Even though most FTLs were in the CD4+CD8+ stage, all FTLs expressed TCR-β mRNA with TCR-γ mRNA, but without TCR-α mRNA or TCR-δ mRNA. One of the FTLs, cFTL53, expressed two kinds of TCR-β mRNA and two kinds of TCR-γ mRNA, but did not express any mRNA of TCR-α or TCR-δ. Both alleles of TCR-β loci were rearranged on cFTL53. cDNA cloning and sequencing analysis showed that one TCR-γ mRNA, Vγ4-Jγ1-Cγ1, and both TCR-β mRNA, Vβ2-Dβ2-Jß2.1 and Vβ19-Dβ2-Jβ2.1-Cβ2, on cFTL53 were in the productive form, while the other TCR-γ mRNA, Vγ1-Jγ4-Cγ4L, was not. Both TCR β-chains and a TCR γ-chain were expressed on cFTL53, making a novel set of TCR-βγ heterodimer. Cross-linking of TCR-βγ heterodimer on cFTL53 resulted in a calcium flux, indicating that TCR-βγ works as a signal transduction receptor. Thus, there are four strange phenomena on FTLs; CD4 and CD8 are expressed without TCR-αβ or TCR-γδ, TCR-β mRNA and TCR-γ mRNA were expressed simultaneously without TCR-α and TCR-δ mRNA on FTLs, the allelic exclusion of TCR-β was destroyed in cFTL53, and a novel set of functional TCR-βγ heterodimer was expressed on cFTL53.

AB - Thymic lymphomas (FTLs) induced by the chemical carcinogen 1-propyl-1-nitrosourea (PNU) in F344 rats showed deviated overexpression of TCR-β, TCR-γ, CD4, and CD8. Even though most FTLs were in the CD4+CD8+ stage, all FTLs expressed TCR-β mRNA with TCR-γ mRNA, but without TCR-α mRNA or TCR-δ mRNA. One of the FTLs, cFTL53, expressed two kinds of TCR-β mRNA and two kinds of TCR-γ mRNA, but did not express any mRNA of TCR-α or TCR-δ. Both alleles of TCR-β loci were rearranged on cFTL53. cDNA cloning and sequencing analysis showed that one TCR-γ mRNA, Vγ4-Jγ1-Cγ1, and both TCR-β mRNA, Vβ2-Dβ2-Jß2.1 and Vβ19-Dβ2-Jβ2.1-Cβ2, on cFTL53 were in the productive form, while the other TCR-γ mRNA, Vγ1-Jγ4-Cγ4L, was not. Both TCR β-chains and a TCR γ-chain were expressed on cFTL53, making a novel set of TCR-βγ heterodimer. Cross-linking of TCR-βγ heterodimer on cFTL53 resulted in a calcium flux, indicating that TCR-βγ works as a signal transduction receptor. Thus, there are four strange phenomena on FTLs; CD4 and CD8 are expressed without TCR-αβ or TCR-γδ, TCR-β mRNA and TCR-γ mRNA were expressed simultaneously without TCR-α and TCR-δ mRNA on FTLs, the allelic exclusion of TCR-β was destroyed in cFTL53, and a novel set of functional TCR-βγ heterodimer was expressed on cFTL53.

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M3 - Article

VL - 159

SP - 748

EP - 756

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -