DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway

Masashi Okada, Yasukazu Hozumi, Kiyoshi Iwazaki, Kentaro Misaki, Mitsuaki Yanagida, Yoshihiko Araki, Takashi Watanabe, Hitoshi Yagisawa, Matthew K. Topham, Kozo Kaibuchi, Kaoru Goto

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Diacylglycerol kinase (DGK) plays an important role in phosphoinositide signaling cascade by regulating the intracellular level of diacylglycerol and phosphatidic acid. The DGK family is involved in various pathophysiological responses that are mediated through unique binding partners in different tissues and cells. In this study, we identified a small GTPase effector protein, IQGAP1, as a novel DGKζ-associated complex protein. A bacterial endotoxin, lipopolysaccharide (LPS), facilitated the complex formation in macrophages. Both proteins co-localized at the edge and phagocytic cup of the cell. Furthermore, RNA interference-mediated knockdown of DGKζ or IQGAP1 impaired LPS-induced Rac1 activation. Primary macrophages derived from DGKζ-/- mice attenuated LPS-induced phagocytosis of bacteria. These results suggest that DGKζ is involved in IQGAP1/Rac1-mediated phagocytosis upon LPS stimulation in macrophages.

Original languageEnglish
Pages (from-to)479-484
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume420
Issue number2
DOIs
Publication statusPublished - 06-04-2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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