Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation

Dominique T. Brandt, Sabrina Marion, Gareth Griffiths, Takashi Watanabe, Kozo Kaibuchi, Robert Grosse

Research output: Contribution to journalArticlepeer-review

137 Citations (Scopus)

Abstract

The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dia1 to spatially define actin polymerization are largely unknown. In this study, we identify the cytoskeletal scaffold protein IQGAP1 as a Dia1-binding protein that is necessary for its subcellular location. IQGAP1 interacts with Dia1 through a region within the Diaphanous inhibitory domain after the RhoA-mediated release of Dia1 auto-inhibition. Both proteins colocalize at the front of migrating cells but also at the actin-rich phagocytic cup in macrophages. We show that IQGAP1 interaction with Dia1 is required for phagocytosis and phagocytic cup formation. Thus, we identify IQGAP1 as a novel component involved in the regulation of phagocytosis by mediating the localization of the actin filament nucleator Dia1.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalJournal of Cell Biology
Volume178
Issue number2
DOIs
Publication statusPublished - 16-07-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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