TY - JOUR
T1 - Diagnostic usefulness of 18F-FAMT PET and L-type amino acid transporter 1 (LAT1) expression in oral squamous cell carcinoma
AU - Nobusawa, Aiko
AU - Kim, Mai
AU - Kaira, Kyoichi
AU - Miyashita, Go
AU - Negishi, Akihide
AU - Oriuchi, Noboru
AU - Higuchi, Tetsuya
AU - Tsushima, Yoshito
AU - Kanai, Yoshikatsu
AU - Yokoo, Satoshi
AU - Oyama, Tetsunari
PY - 2013/10
Y1 - 2013/10
N2 - Purpose: l-[3-18F]-α-Methyltyrosine (18F-FAMT) was developed as an amino acid tracer for PET imaging to provide better specificity than 2-[18F]fluoro-2-deoxy-d-glucose (18F-FDG) PET for cancer diagnosis. We investigated the diagnostic usefulness of 18F-FAMT in oral squamous cell carcinoma (OSCC). The correlation between tumour uptake of 18F-FAMT and L-type amino acid transporter 1 (LAT1) expression was determined. Methods: The study group comprised 68 OSCC patients who underwent both 18F-FAMT and 18F-FDG PET. Resected tumour sections were stained by immunohistochemistry for LAT1, CD98 and Ki-67, and microvessel density was determined in terms of CD34 and p53 expression. Results: The sensitivity of primary tumour detection by 18F-FAMT and 18F-FDG PET was 98 % and 100 %, respectively. The sensitivity, specificity and accuracy of 18F-FAMT PET for detecting malignant lymph nodes were 68 %, 99 % and 97 %, respectively, and equivalent values for 18F-FDG PET were 84 %, 94 % and 94 %, respectively. The specificity and accuracy of 18F-FAMT were significantly higher than those of 18F-FDG. The uptake of 18F-FAMT was significantly correlated with LAT1 expression, cell proliferation and advanced stage. The expression of LAT1 in OSCC cells was closely correlated with CD98 levels, cell proliferation and angiogenesis. Conclusion: 18F-FAMT PET showed higher specificity for detecting malignant lesions than 18F-FDG PET. The uptake of 18F-FAMT by OSCC cells can be determined by the presence of LAT1 expression and tumour cell proliferation.
AB - Purpose: l-[3-18F]-α-Methyltyrosine (18F-FAMT) was developed as an amino acid tracer for PET imaging to provide better specificity than 2-[18F]fluoro-2-deoxy-d-glucose (18F-FDG) PET for cancer diagnosis. We investigated the diagnostic usefulness of 18F-FAMT in oral squamous cell carcinoma (OSCC). The correlation between tumour uptake of 18F-FAMT and L-type amino acid transporter 1 (LAT1) expression was determined. Methods: The study group comprised 68 OSCC patients who underwent both 18F-FAMT and 18F-FDG PET. Resected tumour sections were stained by immunohistochemistry for LAT1, CD98 and Ki-67, and microvessel density was determined in terms of CD34 and p53 expression. Results: The sensitivity of primary tumour detection by 18F-FAMT and 18F-FDG PET was 98 % and 100 %, respectively. The sensitivity, specificity and accuracy of 18F-FAMT PET for detecting malignant lymph nodes were 68 %, 99 % and 97 %, respectively, and equivalent values for 18F-FDG PET were 84 %, 94 % and 94 %, respectively. The specificity and accuracy of 18F-FAMT were significantly higher than those of 18F-FDG. The uptake of 18F-FAMT was significantly correlated with LAT1 expression, cell proliferation and advanced stage. The expression of LAT1 in OSCC cells was closely correlated with CD98 levels, cell proliferation and angiogenesis. Conclusion: 18F-FAMT PET showed higher specificity for detecting malignant lesions than 18F-FDG PET. The uptake of 18F-FAMT by OSCC cells can be determined by the presence of LAT1 expression and tumour cell proliferation.
KW - LAT1
KW - Oral cancer
KW - Squamous cell carcinoma
UR - https://www.scopus.com/pages/publications/84885423533
UR - https://www.scopus.com/inward/citedby.url?scp=84885423533&partnerID=8YFLogxK
U2 - 10.1007/s00259-013-2477-9
DO - 10.1007/s00259-013-2477-9
M3 - Article
C2 - 23801167
AN - SCOPUS:84885423533
SN - 1619-7070
VL - 40
SP - 1692
EP - 1700
JO - European Journal of Nuclear Medicine and Molecular Imaging
JF - European Journal of Nuclear Medicine and Molecular Imaging
IS - 11
ER -
