Dietary Aloe Vera gel powder and extract inhibit azoxymethaneinduced colorectal aberrant crypt foci in mice fed a highfat diet

Takeshi Chihara, Kan Shimpo, Takaaki Kaneko, Hidehiko Beppu, Takashi Higashiguchi, Shigeru Sonoda, Miyuki Tanaka, Muneo Yamada, Fumiaki Abe

Research output: Contribution to journalArticle

Abstract

Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

Original languageEnglish
Pages (from-to)683-687
Number of pages5
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number2
DOIs
Publication statusPublished - 01-01-2015

Fingerprint

Aberrant Crypt Foci
Powders
Diet
High Fat Diet
Aloe
Sterols
Aloe vera gel
Intestinal Polyps
Azoxymethane

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Chihara, Takeshi ; Shimpo, Kan ; Kaneko, Takaaki ; Beppu, Hidehiko ; Higashiguchi, Takashi ; Sonoda, Shigeru ; Tanaka, Miyuki ; Yamada, Muneo ; Abe, Fumiaki. / Dietary Aloe Vera gel powder and extract inhibit azoxymethaneinduced colorectal aberrant crypt foci in mice fed a highfat diet. In: Asian Pacific Journal of Cancer Prevention. 2015 ; Vol. 16, No. 2. pp. 683-687.
@article{2eb653c1425e4b7c847bf0abaf4d8294,
title = "Dietary Aloe Vera gel powder and extract inhibit azoxymethaneinduced colorectal aberrant crypt foci in mice fed a highfat diet",
abstract = "Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5{\%} carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3{\%} or 1{\%} AVGP, and HFDC containing 0.0125{\%} (H-) or 0.00375{\%} (L-) AVGE. The number of ACF was significantly lower in mice given 3{\%} AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3{\%} AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3{\%} AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.",
author = "Takeshi Chihara and Kan Shimpo and Takaaki Kaneko and Hidehiko Beppu and Takashi Higashiguchi and Shigeru Sonoda and Miyuki Tanaka and Muneo Yamada and Fumiaki Abe",
year = "2015",
month = "1",
day = "1",
doi = "10.7314/APJCP.2015.16.2.683",
language = "English",
volume = "16",
pages = "683--687",
journal = "Asian Pacific Journal of Cancer Prevention",
issn = "1513-7368",
publisher = "Asian Pacific Organization for Cancer Prevention",
number = "2",

}

Dietary Aloe Vera gel powder and extract inhibit azoxymethaneinduced colorectal aberrant crypt foci in mice fed a highfat diet. / Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki.

In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 2, 01.01.2015, p. 683-687.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dietary Aloe Vera gel powder and extract inhibit azoxymethaneinduced colorectal aberrant crypt foci in mice fed a highfat diet

AU - Chihara, Takeshi

AU - Shimpo, Kan

AU - Kaneko, Takaaki

AU - Beppu, Hidehiko

AU - Higashiguchi, Takashi

AU - Sonoda, Shigeru

AU - Tanaka, Miyuki

AU - Yamada, Muneo

AU - Abe, Fumiaki

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

AB - Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

UR - http://www.scopus.com/inward/record.url?scp=84925452749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925452749&partnerID=8YFLogxK

U2 - 10.7314/APJCP.2015.16.2.683

DO - 10.7314/APJCP.2015.16.2.683

M3 - Article

VL - 16

SP - 683

EP - 687

JO - Asian Pacific Journal of Cancer Prevention

JF - Asian Pacific Journal of Cancer Prevention

SN - 1513-7368

IS - 2

ER -