Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women

Ryosuke Fujii, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Genki Mizuno, Yoshiki Tsuboi, Koji Ohashi, Hiroaki Ishikawa, Yoshitaka Ando, Chiharu Hagiwara, Keisuke Maeda, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: Dietary intake of vegetables is one of the key lifestyle factors associated with preventing cancer and cardiovascular disease (CVD). Although previous studies have provided evidence that dietary factors can alter global DNA methylation levels in humans, little work has been conducted on dietary factors influencing methylation levels of specific genes associated with CVD. The aim of this study was to examine whether dietary intake of vegetables was associated with adenosine triphosphate-binding membrane cassette transporter A1 (ABCA1) DNA methylation levels in leukocytes in a Japanese population. Methods: This cross-sectional study included 279 Japanese adults (125 men, 154 women) without any clinical history of cancer, stroke, or ischemic heart disease. ABCA1 DNA methylation levels in leukocytes were measured using a pyrosequencing method. Information on dietary vegetable intake was obtained from the validated food frequency questionnaire. Results: Mean ABCA1 DNA methylation levels in men and women were 35.6% ± 6.5% and 36.9% ± 6.7%, respectively. In women, multivariable linear regression analysis showed that the highest intake group for dietary vegetable intake (carrot, broccoli, pumpkin, and all vegetables) showed significantly lower levels of ABCA1 DNA methylation than the lowest intake group (P = 0.04, <0.001, 0.001, and 0.02, respectively). No significant association was observed between dietary intake of vegetables and DNA methylation levels in men. Conclusions: High dietary intake of vegetables was associated with decreased ABCA1 DNA methylation levels in Japanese women. This may contribute to a better understanding of the protective effects of dietary vegetable intake on CVD.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalNutrition
Volume65
DOIs
Publication statusPublished - 09-2019

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DNA Methylation
Vegetables
Carrier Proteins
Adenosine Triphosphate
Cardiovascular Diseases
Leukocytes
Cucurbita
Daucus carota
ATP-Binding Cassette Transporters
Membrane Transport Proteins
Brassica
Methylation
Myocardial Ischemia
Life Style
Linear Models
Neoplasms
Cross-Sectional Studies
Stroke
Regression Analysis
Food

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Fujii, Ryosuke ; Yamada, Hiroya ; Munetsuna, Eiji ; Yamazaki, Mirai ; Mizuno, Genki ; Tsuboi, Yoshiki ; Ohashi, Koji ; Ishikawa, Hiroaki ; Ando, Yoshitaka ; Hagiwara, Chiharu ; Maeda, Keisuke ; Hashimoto, Shuji ; Hamajima, Nobuyuki ; Suzuki, Koji. / Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women. In: Nutrition. 2019 ; Vol. 65. pp. 1-5.
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title = "Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women",
abstract = "Objective: Dietary intake of vegetables is one of the key lifestyle factors associated with preventing cancer and cardiovascular disease (CVD). Although previous studies have provided evidence that dietary factors can alter global DNA methylation levels in humans, little work has been conducted on dietary factors influencing methylation levels of specific genes associated with CVD. The aim of this study was to examine whether dietary intake of vegetables was associated with adenosine triphosphate-binding membrane cassette transporter A1 (ABCA1) DNA methylation levels in leukocytes in a Japanese population. Methods: This cross-sectional study included 279 Japanese adults (125 men, 154 women) without any clinical history of cancer, stroke, or ischemic heart disease. ABCA1 DNA methylation levels in leukocytes were measured using a pyrosequencing method. Information on dietary vegetable intake was obtained from the validated food frequency questionnaire. Results: Mean ABCA1 DNA methylation levels in men and women were 35.6{\%} ± 6.5{\%} and 36.9{\%} ± 6.7{\%}, respectively. In women, multivariable linear regression analysis showed that the highest intake group for dietary vegetable intake (carrot, broccoli, pumpkin, and all vegetables) showed significantly lower levels of ABCA1 DNA methylation than the lowest intake group (P = 0.04, <0.001, 0.001, and 0.02, respectively). No significant association was observed between dietary intake of vegetables and DNA methylation levels in men. Conclusions: High dietary intake of vegetables was associated with decreased ABCA1 DNA methylation levels in Japanese women. This may contribute to a better understanding of the protective effects of dietary vegetable intake on CVD.",
author = "Ryosuke Fujii and Hiroya Yamada and Eiji Munetsuna and Mirai Yamazaki and Genki Mizuno and Yoshiki Tsuboi and Koji Ohashi and Hiroaki Ishikawa and Yoshitaka Ando and Chiharu Hagiwara and Keisuke Maeda and Shuji Hashimoto and Nobuyuki Hamajima and Koji Suzuki",
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Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women. / Fujii, Ryosuke; Yamada, Hiroya; Munetsuna, Eiji; Yamazaki, Mirai; Mizuno, Genki; Tsuboi, Yoshiki; Ohashi, Koji; Ishikawa, Hiroaki; Ando, Yoshitaka; Hagiwara, Chiharu; Maeda, Keisuke; Hashimoto, Shuji; Hamajima, Nobuyuki; Suzuki, Koji.

In: Nutrition, Vol. 65, 09.2019, p. 1-5.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women

AU - Fujii, Ryosuke

AU - Yamada, Hiroya

AU - Munetsuna, Eiji

AU - Yamazaki, Mirai

AU - Mizuno, Genki

AU - Tsuboi, Yoshiki

AU - Ohashi, Koji

AU - Ishikawa, Hiroaki

AU - Ando, Yoshitaka

AU - Hagiwara, Chiharu

AU - Maeda, Keisuke

AU - Hashimoto, Shuji

AU - Hamajima, Nobuyuki

AU - Suzuki, Koji

PY - 2019/9

Y1 - 2019/9

N2 - Objective: Dietary intake of vegetables is one of the key lifestyle factors associated with preventing cancer and cardiovascular disease (CVD). Although previous studies have provided evidence that dietary factors can alter global DNA methylation levels in humans, little work has been conducted on dietary factors influencing methylation levels of specific genes associated with CVD. The aim of this study was to examine whether dietary intake of vegetables was associated with adenosine triphosphate-binding membrane cassette transporter A1 (ABCA1) DNA methylation levels in leukocytes in a Japanese population. Methods: This cross-sectional study included 279 Japanese adults (125 men, 154 women) without any clinical history of cancer, stroke, or ischemic heart disease. ABCA1 DNA methylation levels in leukocytes were measured using a pyrosequencing method. Information on dietary vegetable intake was obtained from the validated food frequency questionnaire. Results: Mean ABCA1 DNA methylation levels in men and women were 35.6% ± 6.5% and 36.9% ± 6.7%, respectively. In women, multivariable linear regression analysis showed that the highest intake group for dietary vegetable intake (carrot, broccoli, pumpkin, and all vegetables) showed significantly lower levels of ABCA1 DNA methylation than the lowest intake group (P = 0.04, <0.001, 0.001, and 0.02, respectively). No significant association was observed between dietary intake of vegetables and DNA methylation levels in men. Conclusions: High dietary intake of vegetables was associated with decreased ABCA1 DNA methylation levels in Japanese women. This may contribute to a better understanding of the protective effects of dietary vegetable intake on CVD.

AB - Objective: Dietary intake of vegetables is one of the key lifestyle factors associated with preventing cancer and cardiovascular disease (CVD). Although previous studies have provided evidence that dietary factors can alter global DNA methylation levels in humans, little work has been conducted on dietary factors influencing methylation levels of specific genes associated with CVD. The aim of this study was to examine whether dietary intake of vegetables was associated with adenosine triphosphate-binding membrane cassette transporter A1 (ABCA1) DNA methylation levels in leukocytes in a Japanese population. Methods: This cross-sectional study included 279 Japanese adults (125 men, 154 women) without any clinical history of cancer, stroke, or ischemic heart disease. ABCA1 DNA methylation levels in leukocytes were measured using a pyrosequencing method. Information on dietary vegetable intake was obtained from the validated food frequency questionnaire. Results: Mean ABCA1 DNA methylation levels in men and women were 35.6% ± 6.5% and 36.9% ± 6.7%, respectively. In women, multivariable linear regression analysis showed that the highest intake group for dietary vegetable intake (carrot, broccoli, pumpkin, and all vegetables) showed significantly lower levels of ABCA1 DNA methylation than the lowest intake group (P = 0.04, <0.001, 0.001, and 0.02, respectively). No significant association was observed between dietary intake of vegetables and DNA methylation levels in men. Conclusions: High dietary intake of vegetables was associated with decreased ABCA1 DNA methylation levels in Japanese women. This may contribute to a better understanding of the protective effects of dietary vegetable intake on CVD.

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