Difference between follistatin isoforms in the inhibition of activin signalling: Activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin

Osamu Hashimoto, Nana Kawasaki, Kunihiro Tsuchida, Shunichi Shimasaki, Takao Hayakawa, Hiromu Sugino

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin- responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced transcriptional response is more potent than that of FS-315. The potency was not influenced by the presence of heparan sulfates, by which FS, in particular FS-288, associates with cell surfaces at a high affinity. Furthermore, FS-288 inhibited the binding of activin to its type II receptor more markedly than did FS-315, as evidenced by surface plasmon resonance and affinity cross-linking experiments. Moreover, the Kd of FS-288 and FS-315 for activin A was estimated to be 46.5 ± 0.37 pM and 432 ± 26 pM, respectively, by surface plasmon resonance experiments. These results indicate that the different potency between the two FS isoforms in the inhibition of activin activities depends on their affinity for activin A. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)565-571
Number of pages7
JournalCellular Signalling
Volume12
Issue number8
DOIs
Publication statusPublished - 01-08-2000

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Follistatin
Activins
Protein Isoforms
Surface Plasmon Resonance
Heparitin Sulfate

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

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title = "Difference between follistatin isoforms in the inhibition of activin signalling: Activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin",
abstract = "We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin- responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced transcriptional response is more potent than that of FS-315. The potency was not influenced by the presence of heparan sulfates, by which FS, in particular FS-288, associates with cell surfaces at a high affinity. Furthermore, FS-288 inhibited the binding of activin to its type II receptor more markedly than did FS-315, as evidenced by surface plasmon resonance and affinity cross-linking experiments. Moreover, the Kd of FS-288 and FS-315 for activin A was estimated to be 46.5 ± 0.37 pM and 432 ± 26 pM, respectively, by surface plasmon resonance experiments. These results indicate that the different potency between the two FS isoforms in the inhibition of activin activities depends on their affinity for activin A. (C) 2000 Elsevier Science Inc.",
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Difference between follistatin isoforms in the inhibition of activin signalling : Activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin. / Hashimoto, Osamu; Kawasaki, Nana; Tsuchida, Kunihiro; Shimasaki, Shunichi; Hayakawa, Takao; Sugino, Hiromu.

In: Cellular Signalling, Vol. 12, No. 8, 01.08.2000, p. 565-571.

Research output: Contribution to journalArticle

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AU - Hashimoto, Osamu

AU - Kawasaki, Nana

AU - Tsuchida, Kunihiro

AU - Shimasaki, Shunichi

AU - Hayakawa, Takao

AU - Sugino, Hiromu

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N2 - We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin- responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced transcriptional response is more potent than that of FS-315. The potency was not influenced by the presence of heparan sulfates, by which FS, in particular FS-288, associates with cell surfaces at a high affinity. Furthermore, FS-288 inhibited the binding of activin to its type II receptor more markedly than did FS-315, as evidenced by surface plasmon resonance and affinity cross-linking experiments. Moreover, the Kd of FS-288 and FS-315 for activin A was estimated to be 46.5 ± 0.37 pM and 432 ± 26 pM, respectively, by surface plasmon resonance experiments. These results indicate that the different potency between the two FS isoforms in the inhibition of activin activities depends on their affinity for activin A. (C) 2000 Elsevier Science Inc.

AB - We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin- responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced transcriptional response is more potent than that of FS-315. The potency was not influenced by the presence of heparan sulfates, by which FS, in particular FS-288, associates with cell surfaces at a high affinity. Furthermore, FS-288 inhibited the binding of activin to its type II receptor more markedly than did FS-315, as evidenced by surface plasmon resonance and affinity cross-linking experiments. Moreover, the Kd of FS-288 and FS-315 for activin A was estimated to be 46.5 ± 0.37 pM and 432 ± 26 pM, respectively, by surface plasmon resonance experiments. These results indicate that the different potency between the two FS isoforms in the inhibition of activin activities depends on their affinity for activin A. (C) 2000 Elsevier Science Inc.

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