Differences in MHC class I genes between strains of rainbow trout (Oncorhynchus mykiss)

Chun Xia, Ikunari Kiryu, Johannes Martinus Dijkstra, Teruo Azuma, Teruyuki Nakanishi, Mitsuru Ototake

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Abstract

In rainbow trout there is only one dominant classical MHC class I locus, Onmy-UBA, for which four very different allelic lineages have been described. The purpose of the present study was to determine if Onmy-UBA polymorphism could be used for strain characterisation. This was performed by lineage-specific PCR investigation of 30 fish, each of the Nikko and Donaldson strains, and by sequence analysis of 25 of the amplified DNA fragments. Two new MHC class I lineages were detected in addition to the four previously described lineages, thus six distinct lineages were observed within the fish examined (Sal-MHCIa*A-F). The distribution of lineages appeared to be strain-specific. For example, the lineage Sal-MHCIa*A was very common in the Nikko strain but could not be detected in the Donaldson strain. Analysis of MHC class I variation may help to elucidate relationships between strains and the roles of MHC alleles in disease resistance.

Original languageEnglish
Pages (from-to)287-301
Number of pages15
JournalFish and Shellfish Immunology
Volume12
Issue number4
DOIs
Publication statusPublished - 01-01-2002

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All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Xia, C., Kiryu, I., Dijkstra, J. M., Azuma, T., Nakanishi, T., & Ototake, M. (2002). Differences in MHC class I genes between strains of rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 12(4), 287-301. https://doi.org/10.1006/fsim.2001.0371