Different distributions of epstein-barr virus early and late gene transcripts within viral replication compartments

Atsuko Sugimoto, Yoshitaka Sato, Teru Kanda, Takayuki Murata, Yohei Narita, Daisuke Kawashima, Hiroshi Kimura, Tatsuya Tsurumi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Productive replication of the Epstein-Barr virus (EBV) occurs in discrete sites in nuclei, called replication compartments, where viral genome DNA synthesis and transcription take place. The replication compartments include subnuclear domains, designated BMRF1 cores, which are highly enriched in the BMRF1 protein. During viral lytic replication, newly synthesized viral DNA genomes are organized around and then stored inside BMRF1 cores. Here, we examined spatial distribution of viral early and late gene mRNAs within replication compartments using confocal laser scanning microscopy and three-dimensional surface reconstruction imaging. EBV early mRNAs were mainly located outside the BMRF1 cores, while viral late mRNAs were identified inside, corresponding well with the fact that late gene transcription is dependent on viral DNA replication. From these results, we speculate that sites for viral early and late gene transcription are separated with reference to BMRF1 cores.

Original languageEnglish
Pages (from-to)6693-6699
Number of pages7
JournalJournal of Virology
Volume87
Issue number12
DOIs
Publication statusPublished - 01-06-2013

Fingerprint

Genetic Transcription
Human herpesvirus 4
Viral DNA
virus replication
Human Herpesvirus 4
transcription (genetics)
Viral Genome
Messenger RNA
Genes
genome
genes
confocal laser scanning microscopy
DNA
DNA replication
DNA Replication
Confocal Microscopy
spatial distribution
image analysis
synthesis
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Sugimoto, Atsuko ; Sato, Yoshitaka ; Kanda, Teru ; Murata, Takayuki ; Narita, Yohei ; Kawashima, Daisuke ; Kimura, Hiroshi ; Tsurumi, Tatsuya. / Different distributions of epstein-barr virus early and late gene transcripts within viral replication compartments. In: Journal of Virology. 2013 ; Vol. 87, No. 12. pp. 6693-6699.
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Sugimoto, A, Sato, Y, Kanda, T, Murata, T, Narita, Y, Kawashima, D, Kimura, H & Tsurumi, T 2013, 'Different distributions of epstein-barr virus early and late gene transcripts within viral replication compartments', Journal of Virology, vol. 87, no. 12, pp. 6693-6699. https://doi.org/10.1128/JVI.00219-13

Different distributions of epstein-barr virus early and late gene transcripts within viral replication compartments. / Sugimoto, Atsuko; Sato, Yoshitaka; Kanda, Teru; Murata, Takayuki; Narita, Yohei; Kawashima, Daisuke; Kimura, Hiroshi; Tsurumi, Tatsuya.

In: Journal of Virology, Vol. 87, No. 12, 01.06.2013, p. 6693-6699.

Research output: Contribution to journalArticle

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