Differential effects of dietary fatty acids on rat liver α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase activity and gene expression

Yukari Egashira, Gen Murotani, Atsushi Tanabe, Kuniaki Saito, Koich Uehara, Akiko Morise, Mayuki Sato, Hiroo Sanada

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Hepatic α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD; formerly termed picolinic carboxylase) [EC4.1.1.45] plays a key role in regulating NAD biosynthesis and the generation of quinolinate (quinolinic acid) from tryptophan. Quinolinate is a potent endogenous excitotoxin of neuronal cells. We previously reported that ingestion of fatty acids by rats leads to a decrease in their hepatic ACMSD activity. However, the mechanism of this phenomenon is not clarified. We previously purified ACMSD and cloned cDNA encoding rat ACMSD. Therefore, in this study, we examined the differential effect of fatty acids on ACMSD mRNA expression by Northern blot. Moreover, we measured quinolinic acid concentration in rats fed on fatty acid. When diets containing 2% level of fatty acid were given to male Sprague-Dawley rats (4 weeks old) for 8 days, long-chain saturated fatty acids and oleic acid did not affect ACMSD mRNA expression in the liver. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) strongly suppressed the liver ACMSD mRNA expression. In rats fed with high linoleic acid diet for 8 days, serum quinolinic acid was significantly increased as compared with the rats fed on a fatty acid-free diet under the condition of the approximately same calorie ingestion. These results suggest that the transcription level of ACMSD is modulated by polyunsaturated fatty acids, and suppressive potency of ACMSD mRNA is n-3 fatty acid family>linoleic acid (n-6 fatty acid)>saturated fatty acid. Moreover, this study provides the information that a high polyunsaturated fatty acid diet affects the production of quinolinic acid in serum by suppressing the ACMSD activity.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1686
Issue number1-2
DOIs
Publication statusPublished - 08-11-2004

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Carboxy-Lyases
Quinolinic Acid
Fatty Acids
Gene Expression
Liver
Diet
Messenger RNA
Linoleic Acid
Unsaturated Fatty Acids
Eating
Eicosapentaenoic Acid
Docosahexaenoic Acids
Omega-3 Fatty Acids
Neurotoxins
Oleic Acid
Serum
Nonesterified Fatty Acids
Tryptophan
Northern Blotting
NAD

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Egashira, Yukari ; Murotani, Gen ; Tanabe, Atsushi ; Saito, Kuniaki ; Uehara, Koich ; Morise, Akiko ; Sato, Mayuki ; Sanada, Hiroo. / Differential effects of dietary fatty acids on rat liver α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase activity and gene expression. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2004 ; Vol. 1686, No. 1-2. pp. 118-124.
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Differential effects of dietary fatty acids on rat liver α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase activity and gene expression. / Egashira, Yukari; Murotani, Gen; Tanabe, Atsushi; Saito, Kuniaki; Uehara, Koich; Morise, Akiko; Sato, Mayuki; Sanada, Hiroo.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1686, No. 1-2, 08.11.2004, p. 118-124.

Research output: Contribution to journalArticle

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T1 - Differential effects of dietary fatty acids on rat liver α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase activity and gene expression

AU - Egashira, Yukari

AU - Murotani, Gen

AU - Tanabe, Atsushi

AU - Saito, Kuniaki

AU - Uehara, Koich

AU - Morise, Akiko

AU - Sato, Mayuki

AU - Sanada, Hiroo

PY - 2004/11/8

Y1 - 2004/11/8

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AB - Hepatic α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD; formerly termed picolinic carboxylase) [EC4.1.1.45] plays a key role in regulating NAD biosynthesis and the generation of quinolinate (quinolinic acid) from tryptophan. Quinolinate is a potent endogenous excitotoxin of neuronal cells. We previously reported that ingestion of fatty acids by rats leads to a decrease in their hepatic ACMSD activity. However, the mechanism of this phenomenon is not clarified. We previously purified ACMSD and cloned cDNA encoding rat ACMSD. Therefore, in this study, we examined the differential effect of fatty acids on ACMSD mRNA expression by Northern blot. Moreover, we measured quinolinic acid concentration in rats fed on fatty acid. When diets containing 2% level of fatty acid were given to male Sprague-Dawley rats (4 weeks old) for 8 days, long-chain saturated fatty acids and oleic acid did not affect ACMSD mRNA expression in the liver. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) strongly suppressed the liver ACMSD mRNA expression. In rats fed with high linoleic acid diet for 8 days, serum quinolinic acid was significantly increased as compared with the rats fed on a fatty acid-free diet under the condition of the approximately same calorie ingestion. These results suggest that the transcription level of ACMSD is modulated by polyunsaturated fatty acids, and suppressive potency of ACMSD mRNA is n-3 fatty acid family>linoleic acid (n-6 fatty acid)>saturated fatty acid. Moreover, this study provides the information that a high polyunsaturated fatty acid diet affects the production of quinolinic acid in serum by suppressing the ACMSD activity.

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