Differential effects of SH-reactive agents on [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonate and [3H]glutamate binding in brain synaptic membranes treated with triton X-100

Kiyokazu Ogita, Tomoko Kouda, Takeo Suzuki, Atsuko Ohkawara, Riyo Enomoto, Toshitaka Nabeshima, Yukio Yoneda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Specific binding of [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), an antagonist highly selective for the N-methyl-d-aspartate (NMDA)-sensitive subclass of the brain excitatory amino acid receptors, was examined in rat brain synaptic membranes treated with a low concentration of Triton X-100 using a filtration assay method. The binding was displaced by l-glutamic acid (Glu) and its analogous amino acids in a concentration-dependent manner at a concentration range of 10 nM to 0.1 mM. Agonists as well as competitive antagonists for the subclass invariably displaced the binding, while noncompetitive antagonists were ineffective as displacers of the binding. Agonists selective for the other subclasses did not affect the binding, d-2-Amino-5-phosphonovaleric and d-2-amino-7-phosphonoheptanoic acids exhibited more potent inhibition of the binding than their respective l-isomers, with the other aminophosphonates being inactive. Preincubation of synaptic membranes with different SH-reactive agents invariably resulted in a significant reduction of [3H]CPP binding, without significantly affecting NMDA-sensitive [3H]Glu binding. These results suggest that a filtration assay method is also applicable to detecting the binding of an antagonist highly selective for the NMDA-sensitive receptors to the NMDA recognition sites, in addition to a centrifugation assay method. Heterogeneity of the NMDA recognition sites is also suggested.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalNeurochemistry International
Volume18
Issue number1
DOIs
Publication statusPublished - 01-01-1991
Externally publishedYes

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Synaptic Membranes
Organophosphonates
Octoxynol
Aspartic Acid
Glutamic Acid
2-amino-7-phosphonoheptanoic acid
Brain
Glutamate Receptors
Centrifugation
Amino Acids
3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

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title = "Differential effects of SH-reactive agents on [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonate and [3H]glutamate binding in brain synaptic membranes treated with triton X-100",
abstract = "Specific binding of [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), an antagonist highly selective for the N-methyl-d-aspartate (NMDA)-sensitive subclass of the brain excitatory amino acid receptors, was examined in rat brain synaptic membranes treated with a low concentration of Triton X-100 using a filtration assay method. The binding was displaced by l-glutamic acid (Glu) and its analogous amino acids in a concentration-dependent manner at a concentration range of 10 nM to 0.1 mM. Agonists as well as competitive antagonists for the subclass invariably displaced the binding, while noncompetitive antagonists were ineffective as displacers of the binding. Agonists selective for the other subclasses did not affect the binding, d-2-Amino-5-phosphonovaleric and d-2-amino-7-phosphonoheptanoic acids exhibited more potent inhibition of the binding than their respective l-isomers, with the other aminophosphonates being inactive. Preincubation of synaptic membranes with different SH-reactive agents invariably resulted in a significant reduction of [3H]CPP binding, without significantly affecting NMDA-sensitive [3H]Glu binding. These results suggest that a filtration assay method is also applicable to detecting the binding of an antagonist highly selective for the NMDA-sensitive receptors to the NMDA recognition sites, in addition to a centrifugation assay method. Heterogeneity of the NMDA recognition sites is also suggested.",
author = "Kiyokazu Ogita and Tomoko Kouda and Takeo Suzuki and Atsuko Ohkawara and Riyo Enomoto and Toshitaka Nabeshima and Yukio Yoneda",
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language = "English",
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Differential effects of SH-reactive agents on [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonate and [3H]glutamate binding in brain synaptic membranes treated with triton X-100. / Ogita, Kiyokazu; Kouda, Tomoko; Suzuki, Takeo; Ohkawara, Atsuko; Enomoto, Riyo; Nabeshima, Toshitaka; Yoneda, Yukio.

In: Neurochemistry International, Vol. 18, No. 1, 01.01.1991, p. 55-62.

Research output: Contribution to journalArticle

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T1 - Differential effects of SH-reactive agents on [3H](±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonate and [3H]glutamate binding in brain synaptic membranes treated with triton X-100

AU - Ogita, Kiyokazu

AU - Kouda, Tomoko

AU - Suzuki, Takeo

AU - Ohkawara, Atsuko

AU - Enomoto, Riyo

AU - Nabeshima, Toshitaka

AU - Yoneda, Yukio

PY - 1991/1/1

Y1 - 1991/1/1

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