Differential expression of caveolin-3 in mouse smooth muscle cells in vivo

Hiroshi Kogo, Shin Ya Ito, Yoshinobu Moritoki, Hiroki Kurahashi, Toyoshi Fujimoto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Expression of caveolin-1 and -3 in mouse smooth muscle cells in vivo was examined by immunohistochemistry. Caveolin-1 was detected in almost all smooth muscles examined, except for the pupillary dilator muscle, whereas caveolin-3 was present only in smooth muscles of some specific tissues. In the eye, the pupillary sphincter muscle was intensely positive for caveolin-3, whereas the ciliary muscle and pupillary dilator muscle were negative. In the gastrointestinal tract, caveolin-3 was detected in the inner circular layer, but not in the outer longitudinal layer. Vascular smooth muscle cells of the resistance-sized artery in the uterus and corpus cavernosum were intensely positive for caveolin-3, whereas those of the aorta were only weakly positive and those of the vena cava were negative. Caveolin-3 was also detected in smooth muscle cells of the urinary bladder, ureter, prostatic vas deferens, and seminal vesicle. The different levels of caveolin-3 expression among various smooth muscle tissues were confirmed by Western blot analysis. Even within the same muscle, the relative expression levels of caveolin-1 and -3 were variable among neighboring cells, suggesting distinct fine regulation of expression of these two caveolins. Moreover, even in the same cell, caveolin-1 and -3 showed different distributions. These results indicate that the two caveolins form distinct caveolae in smooth muscles, and that caveolin-1 and -3 serve different functions. Their differential expression may therefore be related to the functional diversity of smooth muscles.

Original languageEnglish
Pages (from-to)291-300
Number of pages10
JournalCell and Tissue Research
Volume324
Issue number2
DOIs
Publication statusPublished - 01-05-2006

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Caveolin 3
Smooth Muscle Myocytes
Caveolin 1
Smooth Muscle
Muscles
Caveolins
Caveolae
Venae Cavae
Vas Deferens
Seminal Vesicles
Ureter
Vascular Smooth Muscle

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Kogo, Hiroshi ; Ito, Shin Ya ; Moritoki, Yoshinobu ; Kurahashi, Hiroki ; Fujimoto, Toyoshi. / Differential expression of caveolin-3 in mouse smooth muscle cells in vivo. In: Cell and Tissue Research. 2006 ; Vol. 324, No. 2. pp. 291-300.
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Differential expression of caveolin-3 in mouse smooth muscle cells in vivo. / Kogo, Hiroshi; Ito, Shin Ya; Moritoki, Yoshinobu; Kurahashi, Hiroki; Fujimoto, Toyoshi.

In: Cell and Tissue Research, Vol. 324, No. 2, 01.05.2006, p. 291-300.

Research output: Contribution to journalArticle

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