Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy - Beneficial protective effect of diltiazem

Yosuke Kato, Mitsunori Iwase, Kenji Takagi, Takao Nishizawa, Hiroaki Kanazawa, Aya Matsushita, Hisashi Umeda, Hideo Izawa, Akiko Noda, Yasuo Koike, Kohzo Nagata, Mitsuhiro Yokota

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Although dilated cardiomyopathic hamsters (TO-2) with mutation of the δ-sarcoglycan gene exhibit histological features of muscular dystrophy, it remains to be elucidated whether both myocardium and skeletal muscle are injured in a similar manner. Methods and Results: The progression of myolysis in both myocardium and skeletal muscle were assessed biochemically and pathologically in TO-2 and F1B control hamsters. Left ventricular (LV) function was assessed by echocardiography and cardiac catheterization. Both the plasma concentration of cardiac troponin T and the plasma activity of α-hydroxybutyrate dehydrogenase (HBD) peaked at 8 weeks of age, and thereafter reduced greatly in TO-2 hamsters. Activity of creatine kinase (CK) in TO-2 hamsters was significantly greater than in controls throughout the observation period. Pathological findings of both nuclear chain and central nuclei in skeletal muscles were observed in TO-2 hamsters throughout the observation period, suggesting regeneration. LV dysfunction was first evident at 8 weeks of age and deteriorated thereafter in TO-2 hamsters. Treatment of TO-2 hamsters with diltiazem from 5 to 8 weeks of age could avert the LV functional deterioration and the increment in α-HBD activity, but CK activity was unchanged. Conclusions: Despite myolysis in skeletal muscle occurring consistently throughout the observation period, cardiac myolysis occurred predominantly in the early phase. These initial cardiac events might involve coronary spasm and/or calcium overload in the myocardium.

Original languageEnglish
Pages (from-to)1497-1502
Number of pages6
JournalCirculation Journal
Volume70
Issue number11
DOIs
Publication statusPublished - 08-11-2006

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Diltiazem
Dilated Cardiomyopathy
Cricetinae
Myocardium
Skeletal Muscle
Hydroxybutyrate Dehydrogenase
Observation
Creatine Kinase
Sarcoglycans
Troponin T
Muscular Dystrophies
Spasm
Left Ventricular Dysfunction
Cardiac Catheterization
Left Ventricular Function
Echocardiography
Regeneration
Calcium
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Kato, Yosuke ; Iwase, Mitsunori ; Takagi, Kenji ; Nishizawa, Takao ; Kanazawa, Hiroaki ; Matsushita, Aya ; Umeda, Hisashi ; Izawa, Hideo ; Noda, Akiko ; Koike, Yasuo ; Nagata, Kohzo ; Yokota, Mitsuhiro. / Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy - Beneficial protective effect of diltiazem. In: Circulation Journal. 2006 ; Vol. 70, No. 11. pp. 1497-1502.
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abstract = "Background: Although dilated cardiomyopathic hamsters (TO-2) with mutation of the δ-sarcoglycan gene exhibit histological features of muscular dystrophy, it remains to be elucidated whether both myocardium and skeletal muscle are injured in a similar manner. Methods and Results: The progression of myolysis in both myocardium and skeletal muscle were assessed biochemically and pathologically in TO-2 and F1B control hamsters. Left ventricular (LV) function was assessed by echocardiography and cardiac catheterization. Both the plasma concentration of cardiac troponin T and the plasma activity of α-hydroxybutyrate dehydrogenase (HBD) peaked at 8 weeks of age, and thereafter reduced greatly in TO-2 hamsters. Activity of creatine kinase (CK) in TO-2 hamsters was significantly greater than in controls throughout the observation period. Pathological findings of both nuclear chain and central nuclei in skeletal muscles were observed in TO-2 hamsters throughout the observation period, suggesting regeneration. LV dysfunction was first evident at 8 weeks of age and deteriorated thereafter in TO-2 hamsters. Treatment of TO-2 hamsters with diltiazem from 5 to 8 weeks of age could avert the LV functional deterioration and the increment in α-HBD activity, but CK activity was unchanged. Conclusions: Despite myolysis in skeletal muscle occurring consistently throughout the observation period, cardiac myolysis occurred predominantly in the early phase. These initial cardiac events might involve coronary spasm and/or calcium overload in the myocardium.",
author = "Yosuke Kato and Mitsunori Iwase and Kenji Takagi and Takao Nishizawa and Hiroaki Kanazawa and Aya Matsushita and Hisashi Umeda and Hideo Izawa and Akiko Noda and Yasuo Koike and Kohzo Nagata and Mitsuhiro Yokota",
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Kato, Y, Iwase, M, Takagi, K, Nishizawa, T, Kanazawa, H, Matsushita, A, Umeda, H, Izawa, H, Noda, A, Koike, Y, Nagata, K & Yokota, M 2006, 'Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy - Beneficial protective effect of diltiazem', Circulation Journal, vol. 70, no. 11, pp. 1497-1502. https://doi.org/10.1253/circj.70.1497

Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy - Beneficial protective effect of diltiazem. / Kato, Yosuke; Iwase, Mitsunori; Takagi, Kenji; Nishizawa, Takao; Kanazawa, Hiroaki; Matsushita, Aya; Umeda, Hisashi; Izawa, Hideo; Noda, Akiko; Koike, Yasuo; Nagata, Kohzo; Yokota, Mitsuhiro.

In: Circulation Journal, Vol. 70, No. 11, 08.11.2006, p. 1497-1502.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential myolysis of myocardium and skeletal muscle in hamsters with dilated cardiomyopathy - Beneficial protective effect of diltiazem

AU - Kato, Yosuke

AU - Iwase, Mitsunori

AU - Takagi, Kenji

AU - Nishizawa, Takao

AU - Kanazawa, Hiroaki

AU - Matsushita, Aya

AU - Umeda, Hisashi

AU - Izawa, Hideo

AU - Noda, Akiko

AU - Koike, Yasuo

AU - Nagata, Kohzo

AU - Yokota, Mitsuhiro

PY - 2006/11/8

Y1 - 2006/11/8

N2 - Background: Although dilated cardiomyopathic hamsters (TO-2) with mutation of the δ-sarcoglycan gene exhibit histological features of muscular dystrophy, it remains to be elucidated whether both myocardium and skeletal muscle are injured in a similar manner. Methods and Results: The progression of myolysis in both myocardium and skeletal muscle were assessed biochemically and pathologically in TO-2 and F1B control hamsters. Left ventricular (LV) function was assessed by echocardiography and cardiac catheterization. Both the plasma concentration of cardiac troponin T and the plasma activity of α-hydroxybutyrate dehydrogenase (HBD) peaked at 8 weeks of age, and thereafter reduced greatly in TO-2 hamsters. Activity of creatine kinase (CK) in TO-2 hamsters was significantly greater than in controls throughout the observation period. Pathological findings of both nuclear chain and central nuclei in skeletal muscles were observed in TO-2 hamsters throughout the observation period, suggesting regeneration. LV dysfunction was first evident at 8 weeks of age and deteriorated thereafter in TO-2 hamsters. Treatment of TO-2 hamsters with diltiazem from 5 to 8 weeks of age could avert the LV functional deterioration and the increment in α-HBD activity, but CK activity was unchanged. Conclusions: Despite myolysis in skeletal muscle occurring consistently throughout the observation period, cardiac myolysis occurred predominantly in the early phase. These initial cardiac events might involve coronary spasm and/or calcium overload in the myocardium.

AB - Background: Although dilated cardiomyopathic hamsters (TO-2) with mutation of the δ-sarcoglycan gene exhibit histological features of muscular dystrophy, it remains to be elucidated whether both myocardium and skeletal muscle are injured in a similar manner. Methods and Results: The progression of myolysis in both myocardium and skeletal muscle were assessed biochemically and pathologically in TO-2 and F1B control hamsters. Left ventricular (LV) function was assessed by echocardiography and cardiac catheterization. Both the plasma concentration of cardiac troponin T and the plasma activity of α-hydroxybutyrate dehydrogenase (HBD) peaked at 8 weeks of age, and thereafter reduced greatly in TO-2 hamsters. Activity of creatine kinase (CK) in TO-2 hamsters was significantly greater than in controls throughout the observation period. Pathological findings of both nuclear chain and central nuclei in skeletal muscles were observed in TO-2 hamsters throughout the observation period, suggesting regeneration. LV dysfunction was first evident at 8 weeks of age and deteriorated thereafter in TO-2 hamsters. Treatment of TO-2 hamsters with diltiazem from 5 to 8 weeks of age could avert the LV functional deterioration and the increment in α-HBD activity, but CK activity was unchanged. Conclusions: Despite myolysis in skeletal muscle occurring consistently throughout the observation period, cardiac myolysis occurred predominantly in the early phase. These initial cardiac events might involve coronary spasm and/or calcium overload in the myocardium.

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