TY - JOUR
T1 - Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL
T2 - Comparisons with the Friedewald equation
AU - Yamashita, Shizuya
AU - Kawase, Ryota
AU - Nakaoka, Hajime
AU - Nakatani, Kazuhiro
AU - Inagaki, Miwako
AU - Yuasa-Kawase, Miyako
AU - Tsubakio-Yamamoto, Kazumi
AU - Sandoval, Jose C.
AU - Masuda, Daisaku
AU - Ohama, Tohru
AU - Nakagawa-Toyama, Yumiko
AU - Matsuyama, Akifumi
AU - Nishida, Makoto
AU - Ishigami, Masato
PY - 2009/12/8
Y1 - 2009/12/8
N2 - Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.
AB - Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.
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U2 - 10.1016/j.cca.2009.09.010
DO - 10.1016/j.cca.2009.09.010
M3 - Article
C2 - 19751710
AN - SCOPUS:70350782328
SN - 0009-8981
VL - 410
SP - 31
EP - 38
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -