Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL

Comparisons with the Friedewald equation

Shizuya Yamashita, Ryota Kawase, Hajime Nakaoka, Kazuhiro Nakatani, Miwako Inagaki, Miyako Yuasa-Kawase, Kazumi Tsubakio-Yamamoto, Jose C. Sandoval, Daisaku Masuda, Tohru Ohama, Yumiko Nakagawa-Toyama, Akifumi Matsuyama, Makoto Nishida, Masato Ishigami

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalClinica Chimica Acta
Volume410
Issue number1-2
DOIs
Publication statusPublished - 08-12-2009
Externally publishedYes

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IDL Lipoproteins
LDL Cholesterol
Assays
Serum
Hyperlipoproteinemia Type III
oxidized low density lipoprotein
low density lipoprotein inhibitor
Electrophoresis
Polyacrylamide Gel Electrophoresis
Clinical laboratories

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Yamashita, Shizuya ; Kawase, Ryota ; Nakaoka, Hajime ; Nakatani, Kazuhiro ; Inagaki, Miwako ; Yuasa-Kawase, Miyako ; Tsubakio-Yamamoto, Kazumi ; Sandoval, Jose C. ; Masuda, Daisaku ; Ohama, Tohru ; Nakagawa-Toyama, Yumiko ; Matsuyama, Akifumi ; Nishida, Makoto ; Ishigami, Masato. / Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL : Comparisons with the Friedewald equation. In: Clinica Chimica Acta. 2009 ; Vol. 410, No. 1-2. pp. 31-38.
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title = "Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL: Comparisons with the Friedewald equation",
abstract = "Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.",
author = "Shizuya Yamashita and Ryota Kawase and Hajime Nakaoka and Kazuhiro Nakatani and Miwako Inagaki and Miyako Yuasa-Kawase and Kazumi Tsubakio-Yamamoto and Sandoval, {Jose C.} and Daisaku Masuda and Tohru Ohama and Yumiko Nakagawa-Toyama and Akifumi Matsuyama and Makoto Nishida and Masato Ishigami",
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Yamashita, S, Kawase, R, Nakaoka, H, Nakatani, K, Inagaki, M, Yuasa-Kawase, M, Tsubakio-Yamamoto, K, Sandoval, JC, Masuda, D, Ohama, T, Nakagawa-Toyama, Y, Matsuyama, A, Nishida, M & Ishigami, M 2009, 'Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL: Comparisons with the Friedewald equation', Clinica Chimica Acta, vol. 410, no. 1-2, pp. 31-38. https://doi.org/10.1016/j.cca.2009.09.010

Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL : Comparisons with the Friedewald equation. / Yamashita, Shizuya; Kawase, Ryota; Nakaoka, Hajime; Nakatani, Kazuhiro; Inagaki, Miwako; Yuasa-Kawase, Miyako; Tsubakio-Yamamoto, Kazumi; Sandoval, Jose C.; Masuda, Daisaku; Ohama, Tohru; Nakagawa-Toyama, Yumiko; Matsuyama, Akifumi; Nishida, Makoto; Ishigami, Masato.

In: Clinica Chimica Acta, Vol. 410, No. 1-2, 08.12.2009, p. 31-38.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL

T2 - Comparisons with the Friedewald equation

AU - Yamashita, Shizuya

AU - Kawase, Ryota

AU - Nakaoka, Hajime

AU - Nakatani, Kazuhiro

AU - Inagaki, Miwako

AU - Yuasa-Kawase, Miyako

AU - Tsubakio-Yamamoto, Kazumi

AU - Sandoval, Jose C.

AU - Masuda, Daisaku

AU - Ohama, Tohru

AU - Nakagawa-Toyama, Yumiko

AU - Matsuyama, Akifumi

AU - Nishida, Makoto

AU - Ishigami, Masato

PY - 2009/12/8

Y1 - 2009/12/8

N2 - Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.

AB - Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.

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U2 - 10.1016/j.cca.2009.09.010

DO - 10.1016/j.cca.2009.09.010

M3 - Article

VL - 410

SP - 31

EP - 38

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -