Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL: Comparisons with the Friedewald equation

Shizuya Yamashita, Ryota Kawase, Hajime Nakaoka, Kazuhiro Nakatani, Miwako Inagaki, Miyako Yuasa-Kawase, Kazumi Tsubakio-Yamamoto, Jose C. Sandoval, Daisaku Masuda, Tohru Ohama, Yumiko Nakagawa-Toyama, Akifumi Matsuyama, Makoto Nishida, Masato Ishigami

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Abstract

Background: In routine clinical laboratory testing and numerous epidemiological studies, LDL-cholesterol (LDL-C) has been estimated commonly using the Friedewald equation. We investigated the relationship between the Friedewald equation and 4 homogeneous assays for LDL-C. Methods: LDL-C was determined by 4 homogeneous assays [liquid selective detergent method: LDL-C (L), selective solubilization method: LDL-C (S), elimination method: LDL-C (E), and enzyme selective protecting method: LDL-C (P)]. Samples with discrepancies between the Friedewald equation and the 4 homogeneous assays for LDL-C were subjected to polyacrylamide gel electrophoresis and the β-quantification method. Results: The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r = 0.963). Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis. LDL-C (S) was most strongly correlated with the β-quantification method even in sera from patients with type III hyperlipoproteinemia. Conclusions: Of the 4 homogeneous assays for LDL-C, LDL-C (S) exhibited the closest correlation with the Friedewald equation and the β-quantification method, thus reflecting the current clinical databases for coronary heart disease.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalClinica Chimica Acta
Volume410
Issue number1-2
DOIs
Publication statusPublished - 08-12-2009

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Yamashita, S., Kawase, R., Nakaoka, H., Nakatani, K., Inagaki, M., Yuasa-Kawase, M., Tsubakio-Yamamoto, K., Sandoval, J. C., Masuda, D., Ohama, T., Nakagawa-Toyama, Y., Matsuyama, A., Nishida, M., & Ishigami, M. (2009). Differential reactivities of four homogeneous assays for LDL-cholesterol in serum to intermediate-density lipoproteins and small dense LDL: Comparisons with the Friedewald equation. Clinica Chimica Acta, 410(1-2), 31-38. https://doi.org/10.1016/j.cca.2009.09.010