Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus

Minemori Watanabe, Hiroshi Arima, Kuriko Fukushima, Motomitsu Goto, Hiroshi Shimizu, Masayuki Hayashi, Ryoichi Banno, Ikuko Sato, Nobuaki Ozaki, Hiroshi Nagasaki, Yutaka Oiso

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Expression of neuropeptide Y (Npy) heteronuclear (hn) RNA, an indicator of gene transcription, was significantly increased in the arcuate nucleus of rats 30 min after insulin injection. Npy hnRNA levels were also increased significantly in response to hypoglycemia in rats in which the hypothalamus was deafferentated, although the absolute levels were significantly lower than in sham-operated rats. Direct effects of lowering glucose levels on Npy gene expression were also confirmed in hypothalamic organotypic cultures. Thus, Npy gene transcription in the arcuate nucleus increases rapidly in response to hypoglycemia, and both direct and indirect inputs are involved in the rapid upregulation.

Original languageEnglish
Pages (from-to)3632-3638
Number of pages7
JournalFEBS Letters
Volume582
Issue number25-26
DOIs
Publication statusPublished - 29-10-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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