Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus

Minemori Watanabe; Hiroshi Arima; Kuriko Fukushima; Motomitsu Goto; Hiroshi Shimizu; Masayuki Hayashi; Ryoichi Banno; Ikuko Sato; Nobuaki Ozaki; Hiroshi Nagasaki; Yutaka Oiso

doi:10.1016/j.febslet.2008.09.045

Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus

Minemori Watanabe, Hiroshi Arima, Kuriko Fukushima, Motomitsu Goto, Hiroshi Shimizu, Masayuki Hayashi, Ryoichi Banno, Ikuko Sato, Nobuaki Ozaki, Hiroshi Nagasaki, Yutaka Oiso

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Expression of neuropeptide Y (Npy) heteronuclear (hn) RNA, an indicator of gene transcription, was significantly increased in the arcuate nucleus of rats 30 min after insulin injection. Npy hnRNA levels were also increased significantly in response to hypoglycemia in rats in which the hypothalamus was deafferentated, although the absolute levels were significantly lower than in sham-operated rats. Direct effects of lowering glucose levels on Npy gene expression were also confirmed in hypothalamic organotypic cultures. Thus, Npy gene transcription in the arcuate nucleus increases rapidly in response to hypoglycemia, and both direct and indirect inputs are involved in the rapid upregulation.

Original language	English
Pages (from-to)	3632-3638
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	25-26
DOIs	https://doi.org/10.1016/j.febslet.2008.09.045
Publication status	Published - 29-10-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.09.045

Cite this

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title = "Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus",

abstract = "Expression of neuropeptide Y (Npy) heteronuclear (hn) RNA, an indicator of gene transcription, was significantly increased in the arcuate nucleus of rats 30 min after insulin injection. Npy hnRNA levels were also increased significantly in response to hypoglycemia in rats in which the hypothalamus was deafferentated, although the absolute levels were significantly lower than in sham-operated rats. Direct effects of lowering glucose levels on Npy gene expression were also confirmed in hypothalamic organotypic cultures. Thus, Npy gene transcription in the arcuate nucleus increases rapidly in response to hypoglycemia, and both direct and indirect inputs are involved in the rapid upregulation.",

author = "Minemori Watanabe and Hiroshi Arima and Kuriko Fukushima and Motomitsu Goto and Hiroshi Shimizu and Masayuki Hayashi and Ryoichi Banno and Ikuko Sato and Nobuaki Ozaki and Hiroshi Nagasaki and Yutaka Oiso",

year = "2008",

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doi = "10.1016/j.febslet.2008.09.045",

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pages = "3632--3638",

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TY - JOUR

T1 - Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus

AU - Watanabe, Minemori

AU - Arima, Hiroshi

AU - Fukushima, Kuriko

AU - Goto, Motomitsu

AU - Shimizu, Hiroshi

AU - Hayashi, Masayuki

AU - Banno, Ryoichi

AU - Sato, Ikuko

AU - Ozaki, Nobuaki

AU - Nagasaki, Hiroshi

AU - Oiso, Yutaka

PY - 2008/10/29

Y1 - 2008/10/29

N2 - Expression of neuropeptide Y (Npy) heteronuclear (hn) RNA, an indicator of gene transcription, was significantly increased in the arcuate nucleus of rats 30 min after insulin injection. Npy hnRNA levels were also increased significantly in response to hypoglycemia in rats in which the hypothalamus was deafferentated, although the absolute levels were significantly lower than in sham-operated rats. Direct effects of lowering glucose levels on Npy gene expression were also confirmed in hypothalamic organotypic cultures. Thus, Npy gene transcription in the arcuate nucleus increases rapidly in response to hypoglycemia, and both direct and indirect inputs are involved in the rapid upregulation.

AB - Expression of neuropeptide Y (Npy) heteronuclear (hn) RNA, an indicator of gene transcription, was significantly increased in the arcuate nucleus of rats 30 min after insulin injection. Npy hnRNA levels were also increased significantly in response to hypoglycemia in rats in which the hypothalamus was deafferentated, although the absolute levels were significantly lower than in sham-operated rats. Direct effects of lowering glucose levels on Npy gene expression were also confirmed in hypothalamic organotypic cultures. Thus, Npy gene transcription in the arcuate nucleus increases rapidly in response to hypoglycemia, and both direct and indirect inputs are involved in the rapid upregulation.

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JO - FEBS Letters

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ER -

Direct and indirect modulation of neuropeptide Y gene expression in response to hypoglycemia in rat arcuate nucleus

Abstract

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