Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Masaru Ihira, Shiho Akimoto, Fumi Miyake, Ayano Fujita, Ken Sugata, Sadao Suga, Masahiro Ohashi, Naoko Nishimura, Takao Ozaki, Yoshizo Asano, Tetsushi Yoshikawa

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43 Citations (Scopus)

Abstract

Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

Original languageEnglish
Pages (from-to)22-26
Number of pages5
JournalJournal of Clinical Virology
Volume39
Issue number1
DOIs
Publication statusPublished - 01-05-2007

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

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    Ihira, M., Akimoto, S., Miyake, F., Fujita, A., Sugata, K., Suga, S., Ohashi, M., Nishimura, N., Ozaki, T., Asano, Y., & Yoshikawa, T. (2007). Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method. Journal of Clinical Virology, 39(1), 22-26. https://doi.org/10.1016/j.jcv.2007.02.001