TY - JOUR
T1 - Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
AU - Ihira, Masaru
AU - Akimoto, Shiho
AU - Miyake, Fumi
AU - Fujita, Ayano
AU - Sugata, Ken
AU - Suga, Sadao
AU - Ohashi, Masahiro
AU - Nishimura, Naoko
AU - Ozaki, Takao
AU - Asano, Yoshizo
AU - Yoshikawa, Tetsushi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for the 21st Century COE Program of Medicine of Fujita Health University and the Open Research Center of Fujita Health University, from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2007/5
Y1 - 2007/5
N2 - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).
AB - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).
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U2 - 10.1016/j.jcv.2007.02.001
DO - 10.1016/j.jcv.2007.02.001
M3 - Article
C2 - 17376739
AN - SCOPUS:34147108243
VL - 39
SP - 22
EP - 26
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 1
ER -