Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Masaru Ihira, Shiho Akimoto, Fumi Miyake, Ayano Fujita, Ken Sugata, Sadao Suga, Masahiro Ohashi, Naoko Nishimura, Takao Ozaki, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

Original languageEnglish
Pages (from-to)22-26
Number of pages5
JournalJournal of Clinical Virology
Volume39
Issue number1
DOIs
Publication statusPublished - 01-05-2007

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Human Herpesvirus 6
DNA
Serum
Herpesviridae Infections
Hot Temperature
Hospital Laboratories
Viral DNA
Exanthema
Fever
Sensitivity and Specificity
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Ihira, Masaru ; Akimoto, Shiho ; Miyake, Fumi ; Fujita, Ayano ; Sugata, Ken ; Suga, Sadao ; Ohashi, Masahiro ; Nishimura, Naoko ; Ozaki, Takao ; Asano, Yoshizo ; Yoshikawa, Tetsushi. / Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method. In: Journal of Clinical Virology. 2007 ; Vol. 39, No. 1. pp. 22-26.
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title = "Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method",
abstract = "Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5{\%}, 95.2{\%}, 94.0{\%}, and 96.4{\%}, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).",
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Ihira, M, Akimoto, S, Miyake, F, Fujita, A, Sugata, K, Suga, S, Ohashi, M, Nishimura, N, Ozaki, T, Asano, Y & Yoshikawa, T 2007, 'Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method', Journal of Clinical Virology, vol. 39, no. 1, pp. 22-26. https://doi.org/10.1016/j.jcv.2007.02.001

Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method. / Ihira, Masaru; Akimoto, Shiho; Miyake, Fumi; Fujita, Ayano; Sugata, Ken; Suga, Sadao; Ohashi, Masahiro; Nishimura, Naoko; Ozaki, Takao; Asano, Yoshizo; Yoshikawa, Tetsushi.

In: Journal of Clinical Virology, Vol. 39, No. 1, 01.05.2007, p. 22-26.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

AU - Ihira, Masaru

AU - Akimoto, Shiho

AU - Miyake, Fumi

AU - Fujita, Ayano

AU - Sugata, Ken

AU - Suga, Sadao

AU - Ohashi, Masahiro

AU - Nishimura, Naoko

AU - Ozaki, Takao

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

AB - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

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