Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Masaru Ihira; Shiho Akimoto; Fumi Miyake; Ayano Fujita; Ken Sugata; Sadao Suga; Masahiro Ohashi; Naoko Nishimura; Takao Ozaki; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jcv.2007.02.001

Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Masaru Ihira
, Shiho Akimoto
, Fumi Miyake
, Ayano Fujita
, Ken Sugata
, Sadao Suga
, Masahiro Ohashi
, Naoko Nishimura
, Takao Ozaki
, Yoshizo Asano
, Tetsushi Yoshikawa

Clinical Science for biological monitoring

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

Original language	English
Pages (from-to)	22-26
Number of pages	5
Journal	Journal of Clinical Virology
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/j.jcv.2007.02.001
Publication status	Published - 05-2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology
Infectious Diseases

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10.1016/j.jcv.2007.02.001

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@article{c481a249b15e45c7a1419e8127150814,

title = "Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method",

abstract = "Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5\%, 95.2\%, 94.0\%, and 96.4\%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).",

author = "Masaru Ihira and Shiho Akimoto and Fumi Miyake and Ayano Fujita and Ken Sugata and Sadao Suga and Masahiro Ohashi and Naoko Nishimura and Takao Ozaki and Yoshizo Asano and Tetsushi Yoshikawa",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for the 21st Century COE Program of Medicine of Fujita Health University and the Open Research Center of Fujita Health University, from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",

year = "2007",

month = may,

doi = "10.1016/j.jcv.2007.02.001",

language = "English",

volume = "39",

pages = "22--26",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

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TY - JOUR

T1 - Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

AU - Ihira, Masaru

AU - Akimoto, Shiho

AU - Miyake, Fumi

AU - Fujita, Ayano

AU - Sugata, Ken

AU - Suga, Sadao

AU - Ohashi, Masahiro

AU - Nishimura, Naoko

AU - Ozaki, Takao

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for the 21st Century COE Program of Medicine of Fujita Health University and the Open Research Center of Fujita Health University, from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2007/5

Y1 - 2007/5

N2 - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

AB - Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively. Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).

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Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Abstract

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