Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira; Hiroko Sugiyama; Yoshihiko Enomoto; Yuki Higashimoto; Ken Sugata; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jviromet.2010.01.025

Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira, Hiroko Sugiyama, Yoshihiko Enomoto, Yuki Higashimoto, Ken Sugata, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Journal of Virological Methods
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2010.01.025
Publication status	Published - 01-07-2010

All Science Journal Classification (ASJC) codes

Virology

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Ihira, M., Sugiyama, H., Enomoto, Y., Higashimoto, Y., Sugata, K., Asano, Y., & Yoshikawa, T. (2010). Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. Journal of Virological Methods, 167(1), 103-106. https://doi.org/10.1016/j.jviromet.2010.01.025

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Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. / Ihira, Masaru; Sugiyama, Hiroko; Enomoto, Yoshihiko; Higashimoto, Yuki; Sugata, Ken; Asano, Yoshizo; Yoshikawa, Tetsushi.

In: Journal of Virological Methods, Vol. 167, No. 1, 01.07.2010, p. 103-106.

Research output: Contribution to journal › Article

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