Abstract
A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.
| Original language | English |
|---|---|
| Pages (from-to) | 103-106 |
| Number of pages | 4 |
| Journal | Journal of Virological Methods |
| Volume | 167 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-07-2010 |
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All Science Journal Classification (ASJC) codes
- Virology
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Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. / Ihira, Masaru; Sugiyama, Hiroko; Enomoto, Yoshihiko; Higashimoto, Yuki; Sugata, Ken; Asano, Yoshizo; Yoshikawa, Tetsushi.
In: Journal of Virological Methods, Vol. 167, No. 1, 01.07.2010, p. 103-106.Research output: Contribution to journal › Article
TY - JOUR
T1 - Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
AU - Ihira, Masaru
AU - Sugiyama, Hiroko
AU - Enomoto, Yoshihiko
AU - Higashimoto, Yuki
AU - Sugata, Ken
AU - Asano, Yoshizo
AU - Yoshikawa, Tetsushi
PY - 2010/7/1
Y1 - 2010/7/1
N2 - A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.
AB - A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.
UR - http://www.scopus.com/inward/record.url?scp=77953027214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953027214&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2010.01.025
DO - 10.1016/j.jviromet.2010.01.025
M3 - Article
VL - 167
SP - 103
EP - 106
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 1
ER -