Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira; Hiroko Sugiyama; Yoshihiko Enomoto; Yuki Higashimoto; Ken Sugata; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jviromet.2010.01.025

Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira, Hiroko Sugiyama, Yoshihiko Enomoto, Yuki Higashimoto, Ken Sugata, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Journal of Virological Methods
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2010.01.025
Publication status	Published - 01-07-2010

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.jviromet.2010.01.025

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abstract = "A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.",

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AU - Ihira, Masaru

AU - Sugiyama, Hiroko

AU - Enomoto, Yoshihiko

AU - Higashimoto, Yuki

AU - Sugata, Ken

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

PY - 2010/7/1

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AB - A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

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Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Abstract

All Science Journal Classification (ASJC) codes

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