Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira, Hiroko Sugiyama, Yoshihiko Enomoto, Yuki Higashimoto, Ken Sugata, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalJournal of Virological Methods
Volume167
Issue number1
DOIs
Publication statusPublished - 01-07-2010

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Human Herpesvirus 6
Hematopoietic Stem Cells
Transplants
DNA
Serum
Herpesviridae Infections
Sensitivity and Specificity
Genes
Transplant Recipients

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.",
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Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. / Ihira, Masaru; Sugiyama, Hiroko; Enomoto, Yoshihiko; Higashimoto, Yuki; Sugata, Ken; Asano, Yoshizo; Yoshikawa, Tetsushi.

In: Journal of Virological Methods, Vol. 167, No. 1, 01.07.2010, p. 103-106.

Research output: Contribution to journalArticle

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AU - Sugata, Ken

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

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