Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira; Hiroko Sugiyama; Yoshihiko Enomoto; Yuki Higashimoto; Ken Sugata; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jviromet.2010.01.025

Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira, Hiroko Sugiyama, Yoshihiko Enomoto, Yuki Higashimoto, Ken Sugata, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Journal of Virological Methods
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2010.01.025
Publication status	Published - 07-2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

Access to Document

10.1016/j.jviromet.2010.01.025

Cite this

@article{38a636bbd7d445c6bc08783d2c1fc4e8,

title = "Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients",

abstract = "A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.",

author = "Masaru Ihira and Hiroko Sugiyama and Yoshihiko Enomoto and Yuki Higashimoto and Ken Sugata and Yoshizo Asano and Tetsushi Yoshikawa",

year = "2010",

month = jul,

doi = "10.1016/j.jviromet.2010.01.025",

language = "English",

volume = "167",

pages = "103--106",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

AU - Ihira, Masaru

AU - Sugiyama, Hiroko

AU - Enomoto, Yoshihiko

AU - Higashimoto, Yuki

AU - Sugata, Ken

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

PY - 2010/7

Y1 - 2010/7

N2 - A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

AB - A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

UR - https://www.scopus.com/pages/publications/77953027214

UR - https://www.scopus.com/inward/citedby.url?scp=77953027214&partnerID=8YFLogxK

U2 - 10.1016/j.jviromet.2010.01.025

DO - 10.1016/j.jviromet.2010.01.025

M3 - Article

C2 - 20138915

AN - SCOPUS:77953027214

SN - 0166-0934

VL - 167

SP - 103

EP - 106

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this