Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

Tetsushi Yoshikawa, Takahiro Matsuo, Yoshiki Kawamura, Masahiro Ohashi, Toshihiro Yonekawa, Hidetoshi Kanda, Tsugunori Notomi, Masaru Ihira

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

Original languageEnglish
Pages (from-to)65-67
Number of pages3
JournalJournal of Virological Methods
Volume201
DOIs
Publication statusPublished - 01-06-2014

Fingerprint

Human Herpesvirus 6
Fever
Serum

All Science Journal Classification (ASJC) codes

  • Virology
  • Medicine(all)

Cite this

Yoshikawa, Tetsushi ; Matsuo, Takahiro ; Kawamura, Yoshiki ; Ohashi, Masahiro ; Yonekawa, Toshihiro ; Kanda, Hidetoshi ; Notomi, Tsugunori ; Ihira, Masaru. / Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents. In: Journal of Virological Methods. 2014 ; Vol. 201. pp. 65-67.
@article{2f32395aee2d4feca61a2cb069a147e5,
title = "Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents",
abstract = "The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0{\%}) and specific (96.0{\%}) for the detection of HHV-6B.",
author = "Tetsushi Yoshikawa and Takahiro Matsuo and Yoshiki Kawamura and Masahiro Ohashi and Toshihiro Yonekawa and Hidetoshi Kanda and Tsugunori Notomi and Masaru Ihira",
year = "2014",
month = "6",
day = "1",
doi = "10.1016/j.jviromet.2014.02.017",
language = "English",
volume = "201",
pages = "65--67",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",

}

Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents. / Yoshikawa, Tetsushi; Matsuo, Takahiro; Kawamura, Yoshiki; Ohashi, Masahiro; Yonekawa, Toshihiro; Kanda, Hidetoshi; Notomi, Tsugunori; Ihira, Masaru.

In: Journal of Virological Methods, Vol. 201, 01.06.2014, p. 65-67.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

AU - Yoshikawa, Tetsushi

AU - Matsuo, Takahiro

AU - Kawamura, Yoshiki

AU - Ohashi, Masahiro

AU - Yonekawa, Toshihiro

AU - Kanda, Hidetoshi

AU - Notomi, Tsugunori

AU - Ihira, Masaru

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

AB - The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

UR - http://www.scopus.com/inward/record.url?scp=84896110180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896110180&partnerID=8YFLogxK

U2 - 10.1016/j.jviromet.2014.02.017

DO - 10.1016/j.jviromet.2014.02.017

M3 - Article

C2 - 24589515

AN - SCOPUS:84896110180

VL - 201

SP - 65

EP - 67

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -