Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

Tetsushi Yoshikawa; Takahiro Matsuo; Yoshiki Kawamura; Masahiro Ohashi; Toshihiro Yonekawa; Hidetoshi Kanda; Tsugunori Notomi; Masaru Ihira

doi:10.1016/j.jviromet.2014.02.017

Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

Tetsushi Yoshikawa, Takahiro Matsuo, Yoshiki Kawamura, Masahiro Ohashi, Toshihiro Yonekawa, Hidetoshi Kanda, Tsugunori Notomi, Masaru Ihira

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

Original language	English
Pages (from-to)	65-67
Number of pages	3
Journal	Journal of Virological Methods
Volume	201
DOIs	https://doi.org/10.1016/j.jviromet.2014.02.017
Publication status	Published - 01-06-2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.jviromet.2014.02.017

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title = "Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents",

abstract = "The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0\%) and specific (96.0\%) for the detection of HHV-6B.",

author = "Tetsushi Yoshikawa and Takahiro Matsuo and Yoshiki Kawamura and Masahiro Ohashi and Toshihiro Yonekawa and Hidetoshi Kanda and Tsugunori Notomi and Masaru Ihira",

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T1 - Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

AU - Yoshikawa, Tetsushi

AU - Matsuo, Takahiro

AU - Kawamura, Yoshiki

AU - Ohashi, Masahiro

AU - Yonekawa, Toshihiro

AU - Kanda, Hidetoshi

AU - Notomi, Tsugunori

AU - Ihira, Masaru

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

AB - The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.

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DO - 10.1016/j.jviromet.2014.02.017

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SN - 0166-0934

VL - 201

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EP - 67

JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents

Abstract

All Science Journal Classification (ASJC) codes

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