Discovery, development, and testing of substrates and inhibitors of pp60c-SRC

Raymond J.A. Budde, John S. Mcmurray, Hideyuki Saya, Gary E. Gallick, Victor A. Levin

Research output: Contribution to journalArticle

Abstract

Currently, there are no specific protein tyrosine kinase inhibitors available. This review summarizes our efforts to develop an active-site-directed inhibitor of pp60c-srv Initial efforts are directed at determining substrate specificity with synthetic peptides and at developing a biological system to test the potential of pp60c-src inhibitors to effectively inhibit the growth of pp60c-src activated cell lines. To meet these goals, we have developed new methods for purifying recombinant pp60C-SRC, assaying tyrosine kinase activity, synthesizing cyclic peptides, and generating random peptide libraries. In addition, we discuss the generation of potential artifacts while using polyhydroxy aromatic compounds as tyrosine kinase inhibitors.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalPharmaceutical Biology
Volume33
Issue numberS1
DOIs
Publication statusPublished - 01-01-1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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