Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC1

Nobuhiko Ohno, Nobuo Terada, Masayuki Komada, Sei Saitoh, Frank Costantini, Virgilio Pace, Paul Georg Germann, Klaus Weber, Hisashi Yamakawa, Osamu Ohara, Shinichi Ohno

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Abstract

Protein 4.1B is a membrane skeletal protein expressed in various organs, and is associated with tumor suppressor in lung cancer-1 (TSLC1) in vitro. Although involvement of 4.1B in the intercellular junctions and tumor-suppression was suggested, some controversial results posed questions to the general tumor-suppressive function of 4.1B and its relation to TSLC1 in vivo. In this study, the expression of 4.1B and its interaction with TSLC1 were examined in rodent adrenal gland, and the involvement of 4.1B in tumorigenesis and the effect of 4.1B deficiency on TSLC1 distribution were also investigated using rodent pheochromocytoma and 4.1B-knockout mice. Although plasmalemmal immunolocalization of 4.1B was shown in chromaffin cells of rodent adrenal medulla, expression of 4.1B was maintained in developed pheochromocytoma, and morphological abnormality or pheochromocytoma generation could not be found in 4.1B-deficient mice. Furthermore, molecular interaction and colocalization of 4.1B and TSLC1 were observed in mouse adrenal gland, but the immunolocalization of TSLC1 along chromaffin cell membranes was not affected in the 4.1B-deficient mice. These results suggest that the function of 4.1B as tumor suppressor might significantly differ among organs and species, and that plasmalemmal retention of TSLC1 would be maintained by molecules other than 4.1B interacting in rodent chromaffin cells.

Original languageEnglish
Pages (from-to)506-515
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1793
Issue number3
DOIs
Publication statusPublished - 01-03-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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    Ohno, N., Terada, N., Komada, M., Saitoh, S., Costantini, F., Pace, V., Germann, P. G., Weber, K., Yamakawa, H., Ohara, O., & Ohno, S. (2009). Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC1. Biochimica et Biophysica Acta - Molecular Cell Research, 1793(3), 506-515. https://doi.org/10.1016/j.bbamcr.2009.01.005