Disruption of the CHO1 gene encoding phosphatidylserine synthase in Saccharomyces cerevisiae

Takeshi Hikiji, Keiji Miura, Kazuhiro Kiyono, Isao Shibuya, Akinori Ohta

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

A Saccharomyces cerevisiae mutant that lacked phosphatidylserine synthase EC 2.7.8.8] (CDP-1,2-diacyl-sn-glycerol: L-serine O-phosphatidyltransferase) completely was constructed by disrupting its structural gene, CHO1. Over two-thirds of its coding region, from the starting to the 200th codon, was replaced with a LEU2 DNA fragment. This new cho1 mutant showed no detectable synthesis of phosphatidylserine but grew slowly in a medium that contained either ethanolamine or choline. These results indicate that phosphatidylserine synthase and most probably phosphatidylserine are dispensable in S. cerevisiae but necessary for its optimal growth. Additional supplementation with myo-inositol raised the cellular content of phosphatidylinositol and improved the growth of the mutant, suggesting the importance of the negative charges of the membrane surface. The CHO1-disrupted mutant, when grown on choline, accumulated phosphatidylethanolamine to a significant level even after extensive dilution of the initial culture. It segregated prototrophic revertants that could synthesize phosphatidylethanolamine without recovery of phosphatidyl serine synthesis. These results imply the presence of a route(s) for the formation of ethanolamine or its phosphorylated derivative in S. cerevisiae.

Original languageEnglish
Pages (from-to)894-900
Number of pages7
JournalJournal of Biochemistry
Volume104
Issue number6
DOIs
Publication statusPublished - 12-1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Disruption of the CHO1 gene encoding phosphatidylserine synthase in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this