TY - JOUR
T1 - Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)
AU - Suzuki, Masahiro
AU - Norizuki, Chihiro
AU - Wachino, Jun ichi
AU - Kawamura, Kumiko
AU - Nagano, Noriyuki
AU - Nagano, Yukiko
AU - Hayashi, Wataru
AU - Kimura, Kouji
AU - Doi, Yohei
AU - Arakawa, Yoshichika
N1 - Funding Information:
This work was supported by grants from the Food Safety Commission, Cabinet Office, Government of Japan (Research Program for Risk Assessment Study on Food Safety, no. 1504 ), JSPS KAKENHI Grant Number JP20K10457 , and AMED under Grant Number JP20fk0108061 .
Publisher Copyright:
© 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases
PY - 2022/4
Y1 - 2022/4
N2 - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.
AB - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.
UR - http://www.scopus.com/inward/record.url?scp=85121151206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85121151206&partnerID=8YFLogxK
U2 - 10.1016/j.jiac.2021.12.003
DO - 10.1016/j.jiac.2021.12.003
M3 - Article
C2 - 34916137
AN - SCOPUS:85121151206
SN - 1341-321X
VL - 28
SP - 473
EP - 479
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
IS - 4
ER -