Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki, Chihiro Norizuki, Jun ichi Wachino, Kumiko Kawamura, Noriyuki Nagano, Yukiko Nagano, Wataru Hayashi, Kouji Kimura, Yohei Doi, Yoshichika Arakawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

Original languageEnglish
Pages (from-to)473-479
Number of pages7
JournalJournal of Infection and Chemotherapy
Volume28
Issue number4
DOIs
Publication statusPublished - 04-2022

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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