TY - JOUR
T1 - Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)
AU - Suzuki, Masahiro
AU - Norizuki, Chihiro
AU - Wachino, Jun ichi
AU - Kawamura, Kumiko
AU - Nagano, Noriyuki
AU - Nagano, Yukiko
AU - Hayashi, Wataru
AU - Kimura, Kouji
AU - Doi, Yohei
AU - Arakawa, Yoshichika
N1 - Publisher Copyright:
© 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases
PY - 2022/4
Y1 - 2022/4
N2 - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.
AB - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.
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U2 - 10.1016/j.jiac.2021.12.003
DO - 10.1016/j.jiac.2021.12.003
M3 - Article
C2 - 34916137
AN - SCOPUS:85121151206
SN - 1341-321X
VL - 28
SP - 473
EP - 479
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
IS - 4
ER -