Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki; Chihiro Norizuki; Jun ichi Wachino; Kumiko Kawamura; Noriyuki Nagano; Yukiko Nagano; Wataru Hayashi; Kouji Kimura; Yohei Doi; Yoshichika Arakawa

doi:10.1016/j.jiac.2021.12.003

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki, Chihiro Norizuki, Jun ichi Wachino, Kumiko Kawamura, Noriyuki Nagano, Yukiko Nagano, Wataru Hayashi, Kouji Kimura, Yohei Doi, Yoshichika Arakawa

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objectives: We aimed to elucidate the relationship among bla_CTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring bla_CTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying bla_CTX-M-1 or bla_CTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying bla_CTX-M-14 or bla_CTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying bla_CTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

Original language	English
Pages (from-to)	473-479
Number of pages	7
Journal	Journal of Infection and Chemotherapy
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/j.jiac.2021.12.003
Publication status	Published - 04-2022
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jiac.2021.12.003

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title = "Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)",

abstract = "Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.",

keywords = "I1 plasmid, OSNAp, bla",

author = "Masahiro Suzuki and Chihiro Norizuki and Wachino, \{Jun ichi\} and Kumiko Kawamura and Noriyuki Nagano and Yukiko Nagano and Wataru Hayashi and Kouji Kimura and Yohei Doi and Yoshichika Arakawa",

note = "Publisher Copyright: {\textcopyright} 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases",

year = "2022",

month = apr,

doi = "10.1016/j.jiac.2021.12.003",

language = "English",

volume = "28",

pages = "473--479",

journal = "Journal of Infection and Chemotherapy",

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}

TY - JOUR

T1 - Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

AU - Suzuki, Masahiro

AU - Norizuki, Chihiro

AU - Wachino, Jun ichi

AU - Kawamura, Kumiko

AU - Nagano, Noriyuki

AU - Nagano, Yukiko

AU - Hayashi, Wataru

AU - Kimura, Kouji

AU - Doi, Yohei

AU - Arakawa, Yoshichika

PY - 2022/4

Y1 - 2022/4

N2 - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

AB - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

KW - I1 plasmid

KW - OSNAp

KW - bla

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U2 - 10.1016/j.jiac.2021.12.003

DO - 10.1016/j.jiac.2021.12.003

M3 - Article

C2 - 34916137

AN - SCOPUS:85121151206

SN - 1341-321X

VL - 28

SP - 473

EP - 479

JO - Journal of Infection and Chemotherapy

JF - Journal of Infection and Chemotherapy

IS - 4

ER -

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Abstract

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UN SDGs

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