Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki; Chihiro Norizuki; Jun ichi Wachino; Kumiko Kawamura; Noriyuki Nagano; Yukiko Nagano; Wataru Hayashi; Kouji Kimura; Yohei Doi; Yoshichika Arakawa

doi:10.1016/j.jiac.2021.12.003

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Masahiro Suzuki
, Chihiro Norizuki
, Jun ichi Wachino
, Kumiko Kawamura
, Noriyuki Nagano
, Yukiko Nagano
, Wataru Hayashi
, Kouji Kimura
, Yohei Doi
, Yoshichika Arakawa

Microbiology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Objectives: We aimed to elucidate the relationship among bla_CTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring bla_CTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying bla_CTX-M-1 or bla_CTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying bla_CTX-M-14 or bla_CTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying bla_CTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

Original language	English
Pages (from-to)	473-479
Number of pages	7
Journal	Journal of Infection and Chemotherapy
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/j.jiac.2021.12.003
Publication status	Published - 04-2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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10.1016/j.jiac.2021.12.003

Cite this

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title = "Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)",

abstract = "Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.",

keywords = "I1 plasmid, OSNAp, bla",

author = "Masahiro Suzuki and Chihiro Norizuki and Wachino, \{Jun ichi\} and Kumiko Kawamura and Noriyuki Nagano and Yukiko Nagano and Wataru Hayashi and Kouji Kimura and Yohei Doi and Yoshichika Arakawa",

note = "Publisher Copyright: {\textcopyright} 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases",

year = "2022",

month = apr,

doi = "10.1016/j.jiac.2021.12.003",

language = "English",

volume = "28",

pages = "473--479",

journal = "Journal of Infection and Chemotherapy",

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}

TY - JOUR

T1 - Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

AU - Suzuki, Masahiro

AU - Norizuki, Chihiro

AU - Wachino, Jun ichi

AU - Kawamura, Kumiko

AU - Nagano, Noriyuki

AU - Nagano, Yukiko

AU - Hayashi, Wataru

AU - Kimura, Kouji

AU - Doi, Yohei

AU - Arakawa, Yoshichika

PY - 2022/4

Y1 - 2022/4

N2 - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

AB - Objectives: We aimed to elucidate the relationship among blaCTX-M-carrying plasmids and their transmission between humans and domestic animals. Methods: Phylogenetic relationship of 90 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum β-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). Results: The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8 belonged to a single lineage, respectively, and were primarily associated with domestic animals especially chickens. On the other hand, plasmids carrying blaCTX-M-14 or blaCTX-M-15, identified from both humans and domestic animals, were distributed in two or more lineages. Conclusion: OSNAp has revealed the phylogenetic relationships and diversity of plasmids carrying blaCTX-M more distinctly than pMLST. The findings suggest that circulation of I1 plasmids between humans and animals may contribute to their diversity.

KW - I1 plasmid

KW - OSNAp

KW - bla

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UR - https://www.scopus.com/pages/publications/85121151206#tab=citedBy

U2 - 10.1016/j.jiac.2021.12.003

DO - 10.1016/j.jiac.2021.12.003

M3 - Article

C2 - 34916137

AN - SCOPUS:85121151206

SN - 1341-321X

VL - 28

SP - 473

EP - 479

JO - Journal of Infection and Chemotherapy

JF - Journal of Infection and Chemotherapy

IS - 4

ER -

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Abstract

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All Science Journal Classification (ASJC) codes

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