Distinct predictive performance of Rac1 and Cdc42 in cell migration

Masataka Yamao, Honda Naoki, Katsuyuki Kunida, Kazuhiro Aoki, Michiyuki Matsuda, Shin Ishii

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


We propose a new computation-based approach for elucidating how signaling molecules are decoded in cell migration. In this approach, we performed FRET time-lapse imaging of Rac1 and Cdc42, members of Rho GTPases which are responsible for cell motility, and quantitatively identified the response functions that describe the conversion from the molecular activities to the morphological changes. Based on the identified response functions, we clarified the profiles of how the morphology spatiotemporally changes in response to local and transient activation of Rac1 and Cdc42, and found that Rac1 and Cdc42 activation triggers laterally propagating membrane protrusion. The response functions were also endowed with property of differentiator, which is beneficial for maintaining sensitivity under adaptation to the mean level of input. Using the response function, we could predict the morphological change from molecular activity, and its predictive performance provides a new quantitative measure of how much the Rho GTPases participate in the cell migration. Interestingly, we discovered distinct predictive performance of Rac1 and Cdc42 depending on the migration modes, indicating that Rac1 and Cdc42 contribute to persistent and random migration, respectively. Thus, our proposed predictive approach enabled us to uncover the hidden information processing rules of Rho GTPases in the cell migration.

Original languageEnglish
Article number17527
JournalScientific reports
Publication statusPublished - 04-12-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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