Distinct profiles of epigenetic evolution between colorectal cancers with and without metastasis

Hai Xing Ju, Byonggu An, Yasuyuki Okamoto, Keiko Shinjo, Yukihide Kanemitsu, Koji Komori, Takashi Hirai, Yasuhiro Shimizu, Tsuyoshi Sano, Akira Sawaki, Masahiro Tajika, Kenji Yamao, Makiko Fujii, Hideki Murakami, Hirotaka Osada, Hidemi Ito, Ichiro Takeuchi, Yoshitaka Sekido, Yutaka Kondo

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Liver metastasis is a fatal step in the progression of colorectal cancer (CRC); however, the epigenetic evolution of this process is largely unknown. To decipher the epigenetic alterations during the development of liver metastasis, the DNA methylation status of 12 genes, including 5 classical CpG island methylator phenotype (CIMP) markers, was analyzed in 62 liver metastases and in 78 primary CRCs (53 stage I-III; 25 stage IV). Genome-wide methylation analysis was also performed in stage I-III CRCs and in paired primary and liver metastatic cancers. Methylation frequencies of MGMT and TIMP3 increased progressively from stage I-III CRCs to liver metastasis (P = 0.043 and P = 0.028, respectively). The CIMP-positive cases showed significantly earlier recurrence of disease than did CIMPnegative cases with liver metastasis (P = 0.030), whereas no such difference was found in stage I-III CRCs. Genomewide analysis revealed that more genes were methylated in stage I-III CRCs than in paired stage IV samples (P = 0.008). Hierarchical cluster analysis showed that stage I-III CRCs and stage IV CRCs were clustered into two distinct subgroups, whereas most paired primary and metastatic cancers showed similar methylation profiles. This analysis revealed distinct methylation profiles between stage I-III CRCs and stage IV CRCs, whichmayreflect differences in epigenetic evolution during progression of the disease. In addition, most methylation status in stage IV CRCs seems to be established before metastasis.

Original languageEnglish
Pages (from-to)1835-1846
Number of pages12
JournalAmerican Journal of Pathology
Issue number4
Publication statusPublished - 04-2011

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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