Distribution of nitric oxide synthase in the human cerebral blood vessels and brain tissues

Hidekazu Tomimoto, Masaki Nishimura, Toshihiko Suenaga, Sinichi Nakamura, Ichiro Akiguchi, Hideaki Wakita, Jun Kimura, Bernd Mayer

Research output: Contribution to journalArticle

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Abstract

The distribution of nitric oxide synthase was investigated in human cerebral blood vessels and brain tissues. NADPH-diaphorase histochemistry, which is a marker for nitric oxide synthase in neurons and endothelial cells, revealed periadventitial nerve fibers in the arteries of the circle of Willis and their cortical branches, as well as the common carotid and subclavian arteries. The fibers were mostly nonvaricose in the periadventitial nerve trunk and were varicose within the adventitia. Patchy reaction products were distributed in the perinuclear region of each endothelial cell. Smooth muscle cells in the tunica media were weakly stained. Staining was particularly intense in regions with atherosclerotic changes, which consist of macrophage infiltration and proliferation of fibroblasts. In the neural parenchyma, two types of NADPH-diaphorase reactive neurons were differentiated. Type I neurons were intensely stained, medium-sized, and bipolar or multipolar. They were distributed in the cerebral cortex and white matter, mostly in the subcortical white matter. Type II neurons were lightly stained, small oval neurons with fine processes and were distributed in the cerebral cortex. Endothelial cells were intensely reactive for NADPH-diaphorase in the arteries, arterioles, and capillaries but weakly in veins. Immunohistochemistry for neural nitric oxide synthase labeled perivascular nerves in the larger arteries and those in the neural parenchyma. Both type I and type II neurons were labeled. Nitric oxide synthase in endothelial cells and the nerve encircling blood vessels further suggests a dual control of cerebral circulation by nitric oxide in human brain.

Original languageEnglish
Pages (from-to)930-938
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume14
Issue number6
DOIs
Publication statusPublished - 01-01-1994

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Nitric Oxide Synthase
Blood Vessels
Neurons
NADPH Dehydrogenase
Brain
Endothelial Cells
Arteries
Cerebral Cortex
Cerebrovascular Circulation
Circle of Willis
Tunica Media
Adventitia
Subclavian Artery
Common Carotid Artery
Arterioles
Nerve Fibers
Smooth Muscle Myocytes
Veins
Nitric Oxide
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Tomimoto, Hidekazu ; Nishimura, Masaki ; Suenaga, Toshihiko ; Nakamura, Sinichi ; Akiguchi, Ichiro ; Wakita, Hideaki ; Kimura, Jun ; Mayer, Bernd. / Distribution of nitric oxide synthase in the human cerebral blood vessels and brain tissues. In: Journal of Cerebral Blood Flow and Metabolism. 1994 ; Vol. 14, No. 6. pp. 930-938.
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Tomimoto, H, Nishimura, M, Suenaga, T, Nakamura, S, Akiguchi, I, Wakita, H, Kimura, J & Mayer, B 1994, 'Distribution of nitric oxide synthase in the human cerebral blood vessels and brain tissues', Journal of Cerebral Blood Flow and Metabolism, vol. 14, no. 6, pp. 930-938. https://doi.org/10.1038/jcbfm.1994.124

Distribution of nitric oxide synthase in the human cerebral blood vessels and brain tissues. / Tomimoto, Hidekazu; Nishimura, Masaki; Suenaga, Toshihiko; Nakamura, Sinichi; Akiguchi, Ichiro; Wakita, Hideaki; Kimura, Jun; Mayer, Bernd.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 14, No. 6, 01.01.1994, p. 930-938.

Research output: Contribution to journalArticle

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AU - Tomimoto, Hidekazu

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N2 - The distribution of nitric oxide synthase was investigated in human cerebral blood vessels and brain tissues. NADPH-diaphorase histochemistry, which is a marker for nitric oxide synthase in neurons and endothelial cells, revealed periadventitial nerve fibers in the arteries of the circle of Willis and their cortical branches, as well as the common carotid and subclavian arteries. The fibers were mostly nonvaricose in the periadventitial nerve trunk and were varicose within the adventitia. Patchy reaction products were distributed in the perinuclear region of each endothelial cell. Smooth muscle cells in the tunica media were weakly stained. Staining was particularly intense in regions with atherosclerotic changes, which consist of macrophage infiltration and proliferation of fibroblasts. In the neural parenchyma, two types of NADPH-diaphorase reactive neurons were differentiated. Type I neurons were intensely stained, medium-sized, and bipolar or multipolar. They were distributed in the cerebral cortex and white matter, mostly in the subcortical white matter. Type II neurons were lightly stained, small oval neurons with fine processes and were distributed in the cerebral cortex. Endothelial cells were intensely reactive for NADPH-diaphorase in the arteries, arterioles, and capillaries but weakly in veins. Immunohistochemistry for neural nitric oxide synthase labeled perivascular nerves in the larger arteries and those in the neural parenchyma. Both type I and type II neurons were labeled. Nitric oxide synthase in endothelial cells and the nerve encircling blood vessels further suggests a dual control of cerebral circulation by nitric oxide in human brain.

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