Distribution of Rho-kinase in the bovine brain

Ryota Hashimoto, Yu Nakamura, Hidetaka Kosako, Mutsuki Amano, Kozo Kaibuchi, Masaki Inagaki, Masatoshi Takeda

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62 Citations (Scopus)


Rho-associated kinase (Rho-kinase) is a serine/threonine protein kinase downstream of the small GTPase Rho, which participates in signaling pathways of many cellular functions. Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in the brain has not been elucidated yet. In this study, we investigated the distribution of Rho-kinase using three antibodies recognizing the different epitopes of Rho-kinase. Rho-kinase was abundantly expressed in the gray matter in comparison with the white matter. Strong immunoreactivity was observed in the pyramidal neurons of the cerebral cortex and hippocampus and in the Purkinje cells of the cerebellum. These results indicate that Rho-kinase is abundantly distributed in neurons and might play an important role in remodeling of neurites.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 24-09-1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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