Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse

Feng Xu, Yusuke Sudo, Sho Sanechika, Junpei Yamashita, Sho Shimaguchi, Shun Ichiro Honda, Chiho Ichinose, Masayo Mori-Kojima, Rieko Nakata, Tadaomi Furuta, Minoru Sakurai, Masahiro Sugimoto, Tomoyoshi Soga, Kazunao Kondo, Hiroshi Ichinose

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Quinonoid dihydropteridine reductase (QDPR) catalyzes the regeneration of tetrahydrobiopterin (BH4), a cofactor for monoamine synthesis, phenylalanine hydroxylation and nitric oxide production. Here, we produced and analyzed a transgenic Qdpr-/- mouse model. Unexpectedly, the BH4 contents in the Qdpr-/- mice were not decreased and even increased in some tissues, whereas those of the oxidized form dihydrobiopterin (BH2) were significantly increased. We demonstrated that unlike the wild-type mice, dihydrofolate reductase regenerated BH4 from BH2 in the mutants. Furthermore, we revealed wide alterations in folate-associated metabolism in the Qdpr-/- mice, which suggests an interconnection between folate and biopterin metabolism in the transgenic mouse model.

Original languageEnglish
Pages (from-to)3924-3931
Number of pages8
JournalFEBS Letters
Volume588
Issue number21
DOIs
Publication statusPublished - 03-11-2014

Fingerprint

Biopterin
Folic Acid
Metabolism
Dihydropteridine Reductase
Hydroxylation
Tetrahydrofolate Dehydrogenase
Transgenic Mice
Phenylalanine
Nitric Oxide
Tissue
Regeneration

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Xu, F., Sudo, Y., Sanechika, S., Yamashita, J., Shimaguchi, S., Honda, S. I., ... Ichinose, H. (2014). Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse. FEBS Letters, 588(21), 3924-3931. https://doi.org/10.1016/j.febslet.2014.09.004
Xu, Feng ; Sudo, Yusuke ; Sanechika, Sho ; Yamashita, Junpei ; Shimaguchi, Sho ; Honda, Shun Ichiro ; Ichinose, Chiho ; Mori-Kojima, Masayo ; Nakata, Rieko ; Furuta, Tadaomi ; Sakurai, Minoru ; Sugimoto, Masahiro ; Soga, Tomoyoshi ; Kondo, Kazunao ; Ichinose, Hiroshi. / Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse. In: FEBS Letters. 2014 ; Vol. 588, No. 21. pp. 3924-3931.
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Xu, F, Sudo, Y, Sanechika, S, Yamashita, J, Shimaguchi, S, Honda, SI, Ichinose, C, Mori-Kojima, M, Nakata, R, Furuta, T, Sakurai, M, Sugimoto, M, Soga, T, Kondo, K & Ichinose, H 2014, 'Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse', FEBS Letters, vol. 588, no. 21, pp. 3924-3931. https://doi.org/10.1016/j.febslet.2014.09.004

Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse. / Xu, Feng; Sudo, Yusuke; Sanechika, Sho; Yamashita, Junpei; Shimaguchi, Sho; Honda, Shun Ichiro; Ichinose, Chiho; Mori-Kojima, Masayo; Nakata, Rieko; Furuta, Tadaomi; Sakurai, Minoru; Sugimoto, Masahiro; Soga, Tomoyoshi; Kondo, Kazunao; Ichinose, Hiroshi.

In: FEBS Letters, Vol. 588, No. 21, 03.11.2014, p. 3924-3931.

Research output: Contribution to journalArticle

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AU - Sudo, Yusuke

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AU - Honda, Shun Ichiro

AU - Ichinose, Chiho

AU - Mori-Kojima, Masayo

AU - Nakata, Rieko

AU - Furuta, Tadaomi

AU - Sakurai, Minoru

AU - Sugimoto, Masahiro

AU - Soga, Tomoyoshi

AU - Kondo, Kazunao

AU - Ichinose, Hiroshi

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Xu F, Sudo Y, Sanechika S, Yamashita J, Shimaguchi S, Honda SI et al. Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse. FEBS Letters. 2014 Nov 3;588(21):3924-3931. https://doi.org/10.1016/j.febslet.2014.09.004