Diversity among bla KPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients

Tracy H. Hazen, Roberta Mettus, Christi L. McElheny, Sarah L. Bowler, Sushma Nagaraj, Yohei Doi, David A. Rasko

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Carbapenem resistant Enterobacteriaceae are a significant public health concern, and genes encoding the Klebsiella pneumoniae carbapenemase (KPC) have contributed to the global spread of carbapenem resistance. In the current study, we used whole-genome sequencing to investigate the diversity of bla KPC-containing plasmids and antimicrobial resistance mechanisms among 26 bla KPC-containing Escherichia coli, and 13 bla KPC-containing Enterobacter asburiae, Enterobacter hormaechei, K. pneumoniae, Klebsiella variicola, Klebsiella michiganensis, and Serratia marcescens strains, which were isolated from the same patients as the bla KPC-containing E. coli. A bla KPC-containing IncN and/or IncFIIK plasmid was identified in 77% (30/39) of the E. coli and other bacterial species analyzed. Complete genome sequencing and comparative analysis of a bla KPC-containing IncN plasmid from one of the E. coli strains demonstrated that this plasmid is present in the K. pneumoniae and S. marcescens strains from this patient, and is conserved among 13 of the E. coli and other bacterial species analyzed. Interestingly, while both IncFIIK and IncN plasmids were prevalent among the strains analyzed, the IncN plasmids were more often identified in multiple bacterial species from the same patients, demonstrating a contribution of this IncN plasmid to the inter-genera dissemination of the bla KPC genes between the E. coli and other bacterial species analyzed.

Original languageEnglish
Article number10291
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 01-12-2018

Fingerprint

Klebsiella pneumoniae
Plasmids
Escherichia coli
Enterobacter
Serratia marcescens
Carbapenems
Klebsiella
carbapenemase
Genome
Enterobacteriaceae
Genes
Public Health

All Science Journal Classification (ASJC) codes

  • General

Cite this

Hazen, Tracy H. ; Mettus, Roberta ; McElheny, Christi L. ; Bowler, Sarah L. ; Nagaraj, Sushma ; Doi, Yohei ; Rasko, David A. / Diversity among bla KPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients. In: Scientific reports. 2018 ; Vol. 8, No. 1.
@article{bbad34ebe1d448bc903e75cd1db71246,
title = "Diversity among bla KPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients",
abstract = "Carbapenem resistant Enterobacteriaceae are a significant public health concern, and genes encoding the Klebsiella pneumoniae carbapenemase (KPC) have contributed to the global spread of carbapenem resistance. In the current study, we used whole-genome sequencing to investigate the diversity of bla KPC-containing plasmids and antimicrobial resistance mechanisms among 26 bla KPC-containing Escherichia coli, and 13 bla KPC-containing Enterobacter asburiae, Enterobacter hormaechei, K. pneumoniae, Klebsiella variicola, Klebsiella michiganensis, and Serratia marcescens strains, which were isolated from the same patients as the bla KPC-containing E. coli. A bla KPC-containing IncN and/or IncFIIK plasmid was identified in 77{\%} (30/39) of the E. coli and other bacterial species analyzed. Complete genome sequencing and comparative analysis of a bla KPC-containing IncN plasmid from one of the E. coli strains demonstrated that this plasmid is present in the K. pneumoniae and S. marcescens strains from this patient, and is conserved among 13 of the E. coli and other bacterial species analyzed. Interestingly, while both IncFIIK and IncN plasmids were prevalent among the strains analyzed, the IncN plasmids were more often identified in multiple bacterial species from the same patients, demonstrating a contribution of this IncN plasmid to the inter-genera dissemination of the bla KPC genes between the E. coli and other bacterial species analyzed.",
author = "Hazen, {Tracy H.} and Roberta Mettus and McElheny, {Christi L.} and Bowler, {Sarah L.} and Sushma Nagaraj and Yohei Doi and Rasko, {David A.}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-28085-7",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Diversity among bla KPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients. / Hazen, Tracy H.; Mettus, Roberta; McElheny, Christi L.; Bowler, Sarah L.; Nagaraj, Sushma; Doi, Yohei; Rasko, David A.

In: Scientific reports, Vol. 8, No. 1, 10291, 01.12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Diversity among bla KPC-containing plasmids in Escherichia coli and other bacterial species isolated from the same patients

AU - Hazen, Tracy H.

AU - Mettus, Roberta

AU - McElheny, Christi L.

AU - Bowler, Sarah L.

AU - Nagaraj, Sushma

AU - Doi, Yohei

AU - Rasko, David A.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Carbapenem resistant Enterobacteriaceae are a significant public health concern, and genes encoding the Klebsiella pneumoniae carbapenemase (KPC) have contributed to the global spread of carbapenem resistance. In the current study, we used whole-genome sequencing to investigate the diversity of bla KPC-containing plasmids and antimicrobial resistance mechanisms among 26 bla KPC-containing Escherichia coli, and 13 bla KPC-containing Enterobacter asburiae, Enterobacter hormaechei, K. pneumoniae, Klebsiella variicola, Klebsiella michiganensis, and Serratia marcescens strains, which were isolated from the same patients as the bla KPC-containing E. coli. A bla KPC-containing IncN and/or IncFIIK plasmid was identified in 77% (30/39) of the E. coli and other bacterial species analyzed. Complete genome sequencing and comparative analysis of a bla KPC-containing IncN plasmid from one of the E. coli strains demonstrated that this plasmid is present in the K. pneumoniae and S. marcescens strains from this patient, and is conserved among 13 of the E. coli and other bacterial species analyzed. Interestingly, while both IncFIIK and IncN plasmids were prevalent among the strains analyzed, the IncN plasmids were more often identified in multiple bacterial species from the same patients, demonstrating a contribution of this IncN plasmid to the inter-genera dissemination of the bla KPC genes between the E. coli and other bacterial species analyzed.

AB - Carbapenem resistant Enterobacteriaceae are a significant public health concern, and genes encoding the Klebsiella pneumoniae carbapenemase (KPC) have contributed to the global spread of carbapenem resistance. In the current study, we used whole-genome sequencing to investigate the diversity of bla KPC-containing plasmids and antimicrobial resistance mechanisms among 26 bla KPC-containing Escherichia coli, and 13 bla KPC-containing Enterobacter asburiae, Enterobacter hormaechei, K. pneumoniae, Klebsiella variicola, Klebsiella michiganensis, and Serratia marcescens strains, which were isolated from the same patients as the bla KPC-containing E. coli. A bla KPC-containing IncN and/or IncFIIK plasmid was identified in 77% (30/39) of the E. coli and other bacterial species analyzed. Complete genome sequencing and comparative analysis of a bla KPC-containing IncN plasmid from one of the E. coli strains demonstrated that this plasmid is present in the K. pneumoniae and S. marcescens strains from this patient, and is conserved among 13 of the E. coli and other bacterial species analyzed. Interestingly, while both IncFIIK and IncN plasmids were prevalent among the strains analyzed, the IncN plasmids were more often identified in multiple bacterial species from the same patients, demonstrating a contribution of this IncN plasmid to the inter-genera dissemination of the bla KPC genes between the E. coli and other bacterial species analyzed.

UR - http://www.scopus.com/inward/record.url?scp=85049641743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049641743&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-28085-7

DO - 10.1038/s41598-018-28085-7

M3 - Article

C2 - 29980699

AN - SCOPUS:85049641743

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10291

ER -