TY - JOUR
T1 - Domain- and site-specific phosphorylation of bovine NF-L by Rho-associated kinase
AU - Hashimoto, Ryota
AU - Nakamura, Yu
AU - Goto, Hidemasa
AU - Wada, Yoshinao
AU - Sakoda, Saburo
AU - Kaibuchi, Kozo
AU - Inagaki, Masaki
AU - Takeda, Masatoshi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scienti®c Research and Cancer Research from the Ministry of Education, Science, Sports, and Culture of Japan, special coordination funds from the Science and Technology Agency of the Government of Japan, and Japan Society of the Promotion of Science Research for the Future.
PY - 1998/4/17
Y1 - 1998/4/17
N2 - Rho-associated kinase (Rho-kinase), the putative target of the small GTP-binding protein Rho, phosphorylated neurofilament protein (NF-L) in vitro with approximately 1 mole phosphate per mole NF-L. Phosphorylated NF-L no longer formed the 10 nm filaments, and NF-L filaments were phosphorylated with a result of nearly complete disassembly. NF-L phosphorylated by Rho-kinase was digested with trypsin, and digested fragments were assigned by MALDI/TOF. Unique phosphorylation sites were found at Ser-26 and Ser-57 in the head domain of NF-L. These results indicate that domain- and site-specific phosphorylation by Rho-kinase may regulate the assembly-disassembly of NF-L filaments.
AB - Rho-associated kinase (Rho-kinase), the putative target of the small GTP-binding protein Rho, phosphorylated neurofilament protein (NF-L) in vitro with approximately 1 mole phosphate per mole NF-L. Phosphorylated NF-L no longer formed the 10 nm filaments, and NF-L filaments were phosphorylated with a result of nearly complete disassembly. NF-L phosphorylated by Rho-kinase was digested with trypsin, and digested fragments were assigned by MALDI/TOF. Unique phosphorylation sites were found at Ser-26 and Ser-57 in the head domain of NF-L. These results indicate that domain- and site-specific phosphorylation by Rho-kinase may regulate the assembly-disassembly of NF-L filaments.
UR - http://www.scopus.com/inward/record.url?scp=0032540127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032540127&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.8446
DO - 10.1006/bbrc.1998.8446
M3 - Article
C2 - 9571164
AN - SCOPUS:0032540127
VL - 245
SP - 407
EP - 411
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -