Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands

Tohru Ohama, Ken ichi Hirano, Zhongyan Zhang, Ryo Aoki, Ken ichi Tsujii, Yumiko Nakagawa-Toyama, Kosuke Tsukamoto, Chiaki Ikegami, Akifumi Matsuyama, Masato Ishigami, Naohiko Sakai, Hisatoyo Hiraoka, Kazumitsu Ueda, Shizuya Yamashita, Yuji Matsuzawa

Research output: Contribution to journalArticle

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Abstract

ATP-binding cassette transporter-1 (ABCA1) is a cause of Tangier disease, which is a familial deficiency of plasma high density lipoproteins (HDL). This molecule is known to be expressed in the multiple tissues and organs including small intestines, liver, and macrophages in the blood vessels. Recent in vivo studies suggested that ABCA1 plays some roles in the flux of cholesterol in the intestines. One of the major questions to understand the roles of ABCA1 in the intestines is the expression pattern in the intestinal epithelial cells. To address this issue, we have investigated the expression and regulation of ABCA1 in Caco-2 cells cultured on Transwell as a model, especially focusing on possible polarized expression of ABCA1. The expression of ABCA1 was up-regulated during the differentiation and under the stimulation of LXR/RXR by the addition of 9-cis-retinoic acid (9-cis-RA) and 22-R-hydroxycholesterol (22-OH). Apolipoprotein-AI-mediated cholesterol efflux was dominant toward the basolateral side of polarized cells when stimulated by 9-cis-RA and 22-OH. The cell surface biotinylation experiment followed by Western blot analyses demonstrated a markedly dominant expression of ABCA1 on the basolateral surface, which was clearly confirmed by the confocal laser scanning microscopy. In conclusion, the present study demonstrates that ABCA1 is dominantly expressed on the basolateral surface of Caco-2 cells tested, suggesting that this molecule may play a role in the basolateral movement of cholesterol at least when stimulated by LXR/RXR ligands.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number3
DOIs
Publication statusPublished - 14-10-2002

Fingerprint

ATP Binding Cassette Transporter 1
Caco-2 Cells
Ligands
Cholesterol
Intestines
Tangier Disease
Biotinylation
Molecules
Macrophages
Apolipoprotein A-I
Blood vessels
HDL Lipoproteins
Confocal Microscopy
Liver
Small Intestine
Blood Vessels
Microscopic examination
Western Blotting
Epithelial Cells
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ohama, Tohru ; Hirano, Ken ichi ; Zhang, Zhongyan ; Aoki, Ryo ; Tsujii, Ken ichi ; Nakagawa-Toyama, Yumiko ; Tsukamoto, Kosuke ; Ikegami, Chiaki ; Matsuyama, Akifumi ; Ishigami, Masato ; Sakai, Naohiko ; Hiraoka, Hisatoyo ; Ueda, Kazumitsu ; Yamashita, Shizuya ; Matsuzawa, Yuji. / Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 296, No. 3. pp. 625-630.
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abstract = "ATP-binding cassette transporter-1 (ABCA1) is a cause of Tangier disease, which is a familial deficiency of plasma high density lipoproteins (HDL). This molecule is known to be expressed in the multiple tissues and organs including small intestines, liver, and macrophages in the blood vessels. Recent in vivo studies suggested that ABCA1 plays some roles in the flux of cholesterol in the intestines. One of the major questions to understand the roles of ABCA1 in the intestines is the expression pattern in the intestinal epithelial cells. To address this issue, we have investigated the expression and regulation of ABCA1 in Caco-2 cells cultured on Transwell as a model, especially focusing on possible polarized expression of ABCA1. The expression of ABCA1 was up-regulated during the differentiation and under the stimulation of LXR/RXR by the addition of 9-cis-retinoic acid (9-cis-RA) and 22-R-hydroxycholesterol (22-OH). Apolipoprotein-AI-mediated cholesterol efflux was dominant toward the basolateral side of polarized cells when stimulated by 9-cis-RA and 22-OH. The cell surface biotinylation experiment followed by Western blot analyses demonstrated a markedly dominant expression of ABCA1 on the basolateral surface, which was clearly confirmed by the confocal laser scanning microscopy. In conclusion, the present study demonstrates that ABCA1 is dominantly expressed on the basolateral surface of Caco-2 cells tested, suggesting that this molecule may play a role in the basolateral movement of cholesterol at least when stimulated by LXR/RXR ligands.",
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Ohama, T, Hirano, KI, Zhang, Z, Aoki, R, Tsujii, KI, Nakagawa-Toyama, Y, Tsukamoto, K, Ikegami, C, Matsuyama, A, Ishigami, M, Sakai, N, Hiraoka, H, Ueda, K, Yamashita, S & Matsuzawa, Y 2002, 'Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands', Biochemical and Biophysical Research Communications, vol. 296, no. 3, pp. 625-630. https://doi.org/10.1016/S0006-291X(02)00853-7

Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands. / Ohama, Tohru; Hirano, Ken ichi; Zhang, Zhongyan; Aoki, Ryo; Tsujii, Ken ichi; Nakagawa-Toyama, Yumiko; Tsukamoto, Kosuke; Ikegami, Chiaki; Matsuyama, Akifumi; Ishigami, Masato; Sakai, Naohiko; Hiraoka, Hisatoyo; Ueda, Kazumitsu; Yamashita, Shizuya; Matsuzawa, Yuji.

In: Biochemical and Biophysical Research Communications, Vol. 296, No. 3, 14.10.2002, p. 625-630.

Research output: Contribution to journalArticle

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T1 - Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands

AU - Ohama, Tohru

AU - Hirano, Ken ichi

AU - Zhang, Zhongyan

AU - Aoki, Ryo

AU - Tsujii, Ken ichi

AU - Nakagawa-Toyama, Yumiko

AU - Tsukamoto, Kosuke

AU - Ikegami, Chiaki

AU - Matsuyama, Akifumi

AU - Ishigami, Masato

AU - Sakai, Naohiko

AU - Hiraoka, Hisatoyo

AU - Ueda, Kazumitsu

AU - Yamashita, Shizuya

AU - Matsuzawa, Yuji

PY - 2002/10/14

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N2 - ATP-binding cassette transporter-1 (ABCA1) is a cause of Tangier disease, which is a familial deficiency of plasma high density lipoproteins (HDL). This molecule is known to be expressed in the multiple tissues and organs including small intestines, liver, and macrophages in the blood vessels. Recent in vivo studies suggested that ABCA1 plays some roles in the flux of cholesterol in the intestines. One of the major questions to understand the roles of ABCA1 in the intestines is the expression pattern in the intestinal epithelial cells. To address this issue, we have investigated the expression and regulation of ABCA1 in Caco-2 cells cultured on Transwell as a model, especially focusing on possible polarized expression of ABCA1. The expression of ABCA1 was up-regulated during the differentiation and under the stimulation of LXR/RXR by the addition of 9-cis-retinoic acid (9-cis-RA) and 22-R-hydroxycholesterol (22-OH). Apolipoprotein-AI-mediated cholesterol efflux was dominant toward the basolateral side of polarized cells when stimulated by 9-cis-RA and 22-OH. The cell surface biotinylation experiment followed by Western blot analyses demonstrated a markedly dominant expression of ABCA1 on the basolateral surface, which was clearly confirmed by the confocal laser scanning microscopy. In conclusion, the present study demonstrates that ABCA1 is dominantly expressed on the basolateral surface of Caco-2 cells tested, suggesting that this molecule may play a role in the basolateral movement of cholesterol at least when stimulated by LXR/RXR ligands.

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