Dopamine receptor DOP1R2 stabilizes appetitive olfactory memory through the Raf/MAPK pathway in Drosophila

Huan Sun, Tomoki Nishioka, Shun Hiramatsu, Shu Kondo, Mutsuki Amano, Kozo Kaibuchi, Toshiharu Ichinose, Hiromu Tanimoto

Research output: Contribution to journalArticle

Abstract

In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.

Original languageEnglish
Pages (from-to)2935-2942
Number of pages8
JournalJournal of Neuroscience
Volume40
Issue number14
DOIs
Publication statusPublished - 01-04-2020

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Sun, H., Nishioka, T., Hiramatsu, S., Kondo, S., Amano, M., Kaibuchi, K., Ichinose, T., & Tanimoto, H. (2020). Dopamine receptor DOP1R2 stabilizes appetitive olfactory memory through the Raf/MAPK pathway in Drosophila. Journal of Neuroscience, 40(14), 2935-2942. https://doi.org/10.1523/JNEUROSCI.1572-19.2020