Downregulation of microRNAs-143 and -145 in B-cell malignancies

Yukihiro Akao, Yoshihito Nakagawa, Yukio Kitade, Tomohiro Kinoshita, Tomoki Naoe

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

Recently, it has been found that inappropriate expression of microRNAs (miRNAs) is strongly associated with carcinogenesis. In this study, we demonstrated that the expression of miRNAs (miRs) -143 and -145, the levels of which were previously shown to be reduced in colon cancers and various kinds of established cancer cell lines, was also decreased in most of the B-cell malignancies examined, including chronic lymphocytic leukemias (CLL), B-cell lymphomas, Epstein-Barr virus (EBV)-transformed B-cell lines, and Burkitt lymphoma cell lines. All samples from 13 CLL patients and eight of nine B-cell lymphoma ones tested exhibited an extremely low expression of miRs-143 and -145. The expression levels of miRs-143 and -145 were consistently low in human Burkitt lymphoma cell lines and were inversely associated with the cell proliferation observed in the EBV-transformed B-cell lines. Moreover, the introduction of either precursor or mature miR-143 and -145 into Raji cells resulted in a significant growth inhibition that occurred in a dose-dependent manner and the target gene of miRNA-143 was determined to be ERK5, as previously reported in human colon cancer DLD-1 cells. Taken together, these findings suggest that miRs-143 and -145 may be useful as biomarkers that differentiate B-cell malignant cells from normal cells and contribute to carcinogenesis in B-cell malignancies by a newly defined mechanism.

Original languageEnglish
Pages (from-to)1914-1920
Number of pages7
JournalCancer Science
Volume98
Issue number12
DOIs
Publication statusPublished - 01-12-2007

Fingerprint

MicroRNAs
B-Lymphocytes
Down-Regulation
B-Cell Lymphoma
Neoplasms
Transformed Cell Line
Burkitt Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Human Herpesvirus 4
Cell Line
Colonic Neoplasms
Carcinogenesis
Biomarkers
Cell Proliferation
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Akao, Yukihiro ; Nakagawa, Yoshihito ; Kitade, Yukio ; Kinoshita, Tomohiro ; Naoe, Tomoki. / Downregulation of microRNAs-143 and -145 in B-cell malignancies. In: Cancer Science. 2007 ; Vol. 98, No. 12. pp. 1914-1920.
@article{c32666537c7442aaab6081fd3dd2facc,
title = "Downregulation of microRNAs-143 and -145 in B-cell malignancies",
abstract = "Recently, it has been found that inappropriate expression of microRNAs (miRNAs) is strongly associated with carcinogenesis. In this study, we demonstrated that the expression of miRNAs (miRs) -143 and -145, the levels of which were previously shown to be reduced in colon cancers and various kinds of established cancer cell lines, was also decreased in most of the B-cell malignancies examined, including chronic lymphocytic leukemias (CLL), B-cell lymphomas, Epstein-Barr virus (EBV)-transformed B-cell lines, and Burkitt lymphoma cell lines. All samples from 13 CLL patients and eight of nine B-cell lymphoma ones tested exhibited an extremely low expression of miRs-143 and -145. The expression levels of miRs-143 and -145 were consistently low in human Burkitt lymphoma cell lines and were inversely associated with the cell proliferation observed in the EBV-transformed B-cell lines. Moreover, the introduction of either precursor or mature miR-143 and -145 into Raji cells resulted in a significant growth inhibition that occurred in a dose-dependent manner and the target gene of miRNA-143 was determined to be ERK5, as previously reported in human colon cancer DLD-1 cells. Taken together, these findings suggest that miRs-143 and -145 may be useful as biomarkers that differentiate B-cell malignant cells from normal cells and contribute to carcinogenesis in B-cell malignancies by a newly defined mechanism.",
author = "Yukihiro Akao and Yoshihito Nakagawa and Yukio Kitade and Tomohiro Kinoshita and Tomoki Naoe",
year = "2007",
month = "12",
day = "1",
doi = "10.1111/j.1349-7006.2007.00618.x",
language = "English",
volume = "98",
pages = "1914--1920",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "12",

}

Downregulation of microRNAs-143 and -145 in B-cell malignancies. / Akao, Yukihiro; Nakagawa, Yoshihito; Kitade, Yukio; Kinoshita, Tomohiro; Naoe, Tomoki.

In: Cancer Science, Vol. 98, No. 12, 01.12.2007, p. 1914-1920.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Downregulation of microRNAs-143 and -145 in B-cell malignancies

AU - Akao, Yukihiro

AU - Nakagawa, Yoshihito

AU - Kitade, Yukio

AU - Kinoshita, Tomohiro

AU - Naoe, Tomoki

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Recently, it has been found that inappropriate expression of microRNAs (miRNAs) is strongly associated with carcinogenesis. In this study, we demonstrated that the expression of miRNAs (miRs) -143 and -145, the levels of which were previously shown to be reduced in colon cancers and various kinds of established cancer cell lines, was also decreased in most of the B-cell malignancies examined, including chronic lymphocytic leukemias (CLL), B-cell lymphomas, Epstein-Barr virus (EBV)-transformed B-cell lines, and Burkitt lymphoma cell lines. All samples from 13 CLL patients and eight of nine B-cell lymphoma ones tested exhibited an extremely low expression of miRs-143 and -145. The expression levels of miRs-143 and -145 were consistently low in human Burkitt lymphoma cell lines and were inversely associated with the cell proliferation observed in the EBV-transformed B-cell lines. Moreover, the introduction of either precursor or mature miR-143 and -145 into Raji cells resulted in a significant growth inhibition that occurred in a dose-dependent manner and the target gene of miRNA-143 was determined to be ERK5, as previously reported in human colon cancer DLD-1 cells. Taken together, these findings suggest that miRs-143 and -145 may be useful as biomarkers that differentiate B-cell malignant cells from normal cells and contribute to carcinogenesis in B-cell malignancies by a newly defined mechanism.

AB - Recently, it has been found that inappropriate expression of microRNAs (miRNAs) is strongly associated with carcinogenesis. In this study, we demonstrated that the expression of miRNAs (miRs) -143 and -145, the levels of which were previously shown to be reduced in colon cancers and various kinds of established cancer cell lines, was also decreased in most of the B-cell malignancies examined, including chronic lymphocytic leukemias (CLL), B-cell lymphomas, Epstein-Barr virus (EBV)-transformed B-cell lines, and Burkitt lymphoma cell lines. All samples from 13 CLL patients and eight of nine B-cell lymphoma ones tested exhibited an extremely low expression of miRs-143 and -145. The expression levels of miRs-143 and -145 were consistently low in human Burkitt lymphoma cell lines and were inversely associated with the cell proliferation observed in the EBV-transformed B-cell lines. Moreover, the introduction of either precursor or mature miR-143 and -145 into Raji cells resulted in a significant growth inhibition that occurred in a dose-dependent manner and the target gene of miRNA-143 was determined to be ERK5, as previously reported in human colon cancer DLD-1 cells. Taken together, these findings suggest that miRs-143 and -145 may be useful as biomarkers that differentiate B-cell malignant cells from normal cells and contribute to carcinogenesis in B-cell malignancies by a newly defined mechanism.

UR - http://www.scopus.com/inward/record.url?scp=35548995316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35548995316&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.2007.00618.x

DO - 10.1111/j.1349-7006.2007.00618.x

M3 - Article

C2 - 17892514

AN - SCOPUS:35548995316

VL - 98

SP - 1914

EP - 1920

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 12

ER -