TY - JOUR
T1 - Downregulation of POLD4 in Calu6 cells results in G1-S blockage through suppression of the Akt-Skp2-p27 pathway
AU - Huang, Qinmiao
AU - Suzuki, Motoshi
AU - Zeng, Yiming
AU - Zhang, Huaping
AU - Yang, Dongyong
AU - Lin, Huihuang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 81141093 ), the Science Foundation of the Fujian Province, China (Grant No. 2013J01290 ) and by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MS).
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Previously, we have shown that downregulation of POLD4 in lung cancer cells delays progression through the G1-S cell cycle transition and leads to increased genomic instability. To date however, detailed molecular mechanisms have not been elucidated to explain how this occurs. In the present study, we found that reduction in POLD4 by siRNA knockdown promoted downregulation of both p-Akt Ser473 and Skp2 as well as upregulation of p27. Furthermore, these protein expression levels were rescued when siRNA-resistant POLD4 was ectopically expressed in the knockdown cells. These data suggest that the POLD4 downregulation is associated with impaired Akt-Skp2-p27 pathway in lung cancer.
AB - Previously, we have shown that downregulation of POLD4 in lung cancer cells delays progression through the G1-S cell cycle transition and leads to increased genomic instability. To date however, detailed molecular mechanisms have not been elucidated to explain how this occurs. In the present study, we found that reduction in POLD4 by siRNA knockdown promoted downregulation of both p-Akt Ser473 and Skp2 as well as upregulation of p27. Furthermore, these protein expression levels were rescued when siRNA-resistant POLD4 was ectopically expressed in the knockdown cells. These data suggest that the POLD4 downregulation is associated with impaired Akt-Skp2-p27 pathway in lung cancer.
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U2 - 10.1016/j.bmcl.2014.02.033
DO - 10.1016/j.bmcl.2014.02.033
M3 - Article
C2 - 24618301
AN - SCOPUS:84897438803
SN - 0960-894X
VL - 24
SP - 1780
EP - 1783
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 7
ER -
