Dual Gas Treatment With Hydrogen and Carbon Monoxide Attenuates Oxidative Stress and Protects From Renal Ischemia-Reperfusion Injury

T. Nishida, T. Hayashi, T. Inamoto, R. Kato, N. Ibuki, Kiyoshi Takahara, T. Takai, Y. Yoshikawa, T. Uchimoto, K. Saito, N. Tanda, J. Kouno, K. Minami, H. Uehara, H. Hirano, H. Nomi, Y. Okada, H. Azuma

Research output: Contribution to journalArticle

Abstract

Background: Hydrogen (H2) and carbon monoxide (CO) gas are both reported to reduce reactive oxygen species and alleviate tissue ischemia-reperfusion (I-R) injury. The present study was conducted to evaluate the effects of a mixture of H2 gas and CO gas (dual gas) in comparison with hydrogen gas (H2: 2%) alone on I-R renal injury (composition of dual gas; N2: 77.8%; O2: 20.9%; H2: 1.30%; CO: 250 parts per million). Methods: Adult male Sprague-Dawley rats (body weight 250–280 g) were divided into 5 groups: (1) sham operation control, (2) dual gas inhalation (dual treatment) without I-R treatment, (3) I-R renal injury, (4) H2 gas alone inhalation (H2 treatment) with I-R renal injury, and (5) dual treatment with I-R renal injury. I-R renal injury was induced by clamping the left renal artery and vein for 45 minutes followed by reperfusion, and then contralateral nephrectomy was performed 2 weeks later. Renal function was markedly decreased at 24 hours after reperfusion, and thereafter the effects of dual gas were assessed by histologic examination and determination of the superoxide radical, together with functional and molecular analyses. Results: Pathologic examination of the kidney of I-R rats revealed severe renal damage. Importantly, cytoprotective effects of the dual treatment in comparison with H2 treatment and I-R renal injury were observed in terms of superoxide radical scavenging activity and histochemical features. Rats given dual treatment and I-R renal injury showed significant decreases in blood urea nitrogen. Increased expression of several inflammatory cytokines (tumor necrosis factor-α, interleukin-6, intracellular adhesion molecule-1, nuclear factor-κB, hypoxia inducible factor-1α, and heme oxygenase-1) was attenuated by the dual treatment. Conclusions: Dual gas inhalation decreases oxidative stress and markedly improves I-R-induced renal injury.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalTransplantation Proceedings
Volume50
Issue number1
DOIs
Publication statusPublished - 01-01-2018

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Carbon Monoxide
Reperfusion Injury
Hydrogen
Oxidative Stress
Gases
Kidney
Reperfusion
Inhalation
Ischemia
Superoxides
Hypoxia-Inducible Factor 1
Heme Oxygenase-1
Renal Veins
Blood Urea Nitrogen
Renal Artery
Nephrectomy
Constriction
Sprague Dawley Rats
Interleukin-6
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Nishida, T. ; Hayashi, T. ; Inamoto, T. ; Kato, R. ; Ibuki, N. ; Takahara, Kiyoshi ; Takai, T. ; Yoshikawa, Y. ; Uchimoto, T. ; Saito, K. ; Tanda, N. ; Kouno, J. ; Minami, K. ; Uehara, H. ; Hirano, H. ; Nomi, H. ; Okada, Y. ; Azuma, H. / Dual Gas Treatment With Hydrogen and Carbon Monoxide Attenuates Oxidative Stress and Protects From Renal Ischemia-Reperfusion Injury. In: Transplantation Proceedings. 2018 ; Vol. 50, No. 1. pp. 250-258.
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abstract = "Background: Hydrogen (H2) and carbon monoxide (CO) gas are both reported to reduce reactive oxygen species and alleviate tissue ischemia-reperfusion (I-R) injury. The present study was conducted to evaluate the effects of a mixture of H2 gas and CO gas (dual gas) in comparison with hydrogen gas (H2: 2{\%}) alone on I-R renal injury (composition of dual gas; N2: 77.8{\%}; O2: 20.9{\%}; H2: 1.30{\%}; CO: 250 parts per million). Methods: Adult male Sprague-Dawley rats (body weight 250–280 g) were divided into 5 groups: (1) sham operation control, (2) dual gas inhalation (dual treatment) without I-R treatment, (3) I-R renal injury, (4) H2 gas alone inhalation (H2 treatment) with I-R renal injury, and (5) dual treatment with I-R renal injury. I-R renal injury was induced by clamping the left renal artery and vein for 45 minutes followed by reperfusion, and then contralateral nephrectomy was performed 2 weeks later. Renal function was markedly decreased at 24 hours after reperfusion, and thereafter the effects of dual gas were assessed by histologic examination and determination of the superoxide radical, together with functional and molecular analyses. Results: Pathologic examination of the kidney of I-R rats revealed severe renal damage. Importantly, cytoprotective effects of the dual treatment in comparison with H2 treatment and I-R renal injury were observed in terms of superoxide radical scavenging activity and histochemical features. Rats given dual treatment and I-R renal injury showed significant decreases in blood urea nitrogen. Increased expression of several inflammatory cytokines (tumor necrosis factor-α, interleukin-6, intracellular adhesion molecule-1, nuclear factor-κB, hypoxia inducible factor-1α, and heme oxygenase-1) was attenuated by the dual treatment. Conclusions: Dual gas inhalation decreases oxidative stress and markedly improves I-R-induced renal injury.",
author = "T. Nishida and T. Hayashi and T. Inamoto and R. Kato and N. Ibuki and Kiyoshi Takahara and T. Takai and Y. Yoshikawa and T. Uchimoto and K. Saito and N. Tanda and J. Kouno and K. Minami and H. Uehara and H. Hirano and H. Nomi and Y. Okada and H. Azuma",
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Nishida, T, Hayashi, T, Inamoto, T, Kato, R, Ibuki, N, Takahara, K, Takai, T, Yoshikawa, Y, Uchimoto, T, Saito, K, Tanda, N, Kouno, J, Minami, K, Uehara, H, Hirano, H, Nomi, H, Okada, Y & Azuma, H 2018, 'Dual Gas Treatment With Hydrogen and Carbon Monoxide Attenuates Oxidative Stress and Protects From Renal Ischemia-Reperfusion Injury', Transplantation Proceedings, vol. 50, no. 1, pp. 250-258. https://doi.org/10.1016/j.transproceed.2017.12.014

Dual Gas Treatment With Hydrogen and Carbon Monoxide Attenuates Oxidative Stress and Protects From Renal Ischemia-Reperfusion Injury. / Nishida, T.; Hayashi, T.; Inamoto, T.; Kato, R.; Ibuki, N.; Takahara, Kiyoshi; Takai, T.; Yoshikawa, Y.; Uchimoto, T.; Saito, K.; Tanda, N.; Kouno, J.; Minami, K.; Uehara, H.; Hirano, H.; Nomi, H.; Okada, Y.; Azuma, H.

In: Transplantation Proceedings, Vol. 50, No. 1, 01.01.2018, p. 250-258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dual Gas Treatment With Hydrogen and Carbon Monoxide Attenuates Oxidative Stress and Protects From Renal Ischemia-Reperfusion Injury

AU - Nishida, T.

AU - Hayashi, T.

AU - Inamoto, T.

AU - Kato, R.

AU - Ibuki, N.

AU - Takahara, Kiyoshi

AU - Takai, T.

AU - Yoshikawa, Y.

AU - Uchimoto, T.

AU - Saito, K.

AU - Tanda, N.

AU - Kouno, J.

AU - Minami, K.

AU - Uehara, H.

AU - Hirano, H.

AU - Nomi, H.

AU - Okada, Y.

AU - Azuma, H.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Hydrogen (H2) and carbon monoxide (CO) gas are both reported to reduce reactive oxygen species and alleviate tissue ischemia-reperfusion (I-R) injury. The present study was conducted to evaluate the effects of a mixture of H2 gas and CO gas (dual gas) in comparison with hydrogen gas (H2: 2%) alone on I-R renal injury (composition of dual gas; N2: 77.8%; O2: 20.9%; H2: 1.30%; CO: 250 parts per million). Methods: Adult male Sprague-Dawley rats (body weight 250–280 g) were divided into 5 groups: (1) sham operation control, (2) dual gas inhalation (dual treatment) without I-R treatment, (3) I-R renal injury, (4) H2 gas alone inhalation (H2 treatment) with I-R renal injury, and (5) dual treatment with I-R renal injury. I-R renal injury was induced by clamping the left renal artery and vein for 45 minutes followed by reperfusion, and then contralateral nephrectomy was performed 2 weeks later. Renal function was markedly decreased at 24 hours after reperfusion, and thereafter the effects of dual gas were assessed by histologic examination and determination of the superoxide radical, together with functional and molecular analyses. Results: Pathologic examination of the kidney of I-R rats revealed severe renal damage. Importantly, cytoprotective effects of the dual treatment in comparison with H2 treatment and I-R renal injury were observed in terms of superoxide radical scavenging activity and histochemical features. Rats given dual treatment and I-R renal injury showed significant decreases in blood urea nitrogen. Increased expression of several inflammatory cytokines (tumor necrosis factor-α, interleukin-6, intracellular adhesion molecule-1, nuclear factor-κB, hypoxia inducible factor-1α, and heme oxygenase-1) was attenuated by the dual treatment. Conclusions: Dual gas inhalation decreases oxidative stress and markedly improves I-R-induced renal injury.

AB - Background: Hydrogen (H2) and carbon monoxide (CO) gas are both reported to reduce reactive oxygen species and alleviate tissue ischemia-reperfusion (I-R) injury. The present study was conducted to evaluate the effects of a mixture of H2 gas and CO gas (dual gas) in comparison with hydrogen gas (H2: 2%) alone on I-R renal injury (composition of dual gas; N2: 77.8%; O2: 20.9%; H2: 1.30%; CO: 250 parts per million). Methods: Adult male Sprague-Dawley rats (body weight 250–280 g) were divided into 5 groups: (1) sham operation control, (2) dual gas inhalation (dual treatment) without I-R treatment, (3) I-R renal injury, (4) H2 gas alone inhalation (H2 treatment) with I-R renal injury, and (5) dual treatment with I-R renal injury. I-R renal injury was induced by clamping the left renal artery and vein for 45 minutes followed by reperfusion, and then contralateral nephrectomy was performed 2 weeks later. Renal function was markedly decreased at 24 hours after reperfusion, and thereafter the effects of dual gas were assessed by histologic examination and determination of the superoxide radical, together with functional and molecular analyses. Results: Pathologic examination of the kidney of I-R rats revealed severe renal damage. Importantly, cytoprotective effects of the dual treatment in comparison with H2 treatment and I-R renal injury were observed in terms of superoxide radical scavenging activity and histochemical features. Rats given dual treatment and I-R renal injury showed significant decreases in blood urea nitrogen. Increased expression of several inflammatory cytokines (tumor necrosis factor-α, interleukin-6, intracellular adhesion molecule-1, nuclear factor-κB, hypoxia inducible factor-1α, and heme oxygenase-1) was attenuated by the dual treatment. Conclusions: Dual gas inhalation decreases oxidative stress and markedly improves I-R-induced renal injury.

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